Keystone Symposia

Herrenhausen Palace Floorplan

This meeting took place in 2023



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NMR in Biological Mechanisms (C3)


Organizer(s) Teresa Carlomagno, Mei Hong and Ichio Shimada
March 27—30, 2023
Herrenhausen Palace • Hannover, Germany
Abstract Deadline: Feb 13, 2023
Scholarship Deadline: Feb 13, 2023
Discounted Registration Deadline: Feb 13, 2023

Organized in collaboration with Volkswagen Foundation

Summary of Meeting:
Among the three major structural biology techniques, NMR spectroscopy bases its strength on the ability of detecting pathways of conformational and dynamic linkages, which are essential for function. Thus, NMR is unique in its power to explain the functional mechanisms of biomolecules and complexes at atomic resolution. In recent years, NMR methodological and technological advances, as well as developments in isotope labeling, have brought high molecular weight complexes into reach of solution-state NMR spectroscopy. In parallel, sophisticated relaxation experiments have allowed the discovery of minor populated states as essential players in the mechanism of function. Last but not least, solid-state NMR spectroscopy has grown to a point where structural and dynamic studies display the same level of resolution and precision as in solution-state NMR, with the advantage of being applicable to molecules of any size. With these capabilities, NMR plays a central role in the description of molecular functions at atomic resolution. In this event, we will review the contribution of NMR spectroscopy to the elucidation of biological mechanisms; in addition we will discuss the future of the field, in particular with respect to the integration of NMR spectroscopy in hybrid structural biology approaches.

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No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 12 hr (am/pm) time


The meeting will begin on Monday, March 27 with registration from 16:00 to 20:00. Conference events conclude on Thursday, March 30 with a closing plenary session from 13:00 to , followed by . We recommend return travel on Friday, March 31 in order to fully experience the meeting.

MONDAY, MARCH 27

16:00—20:00
Arrival and Registration

Grand Mussmann Hotel, Tivoli Room

TUESDAY, MARCH 28

09:00—09:10
Welcoming Remarks (Organizers)

Auditorium
09:10—12:00
Supra-Molecular Machines and Assemblies
Opening Remarks by Oliver Grewe, Volkswagen Foundation

Auditorium
* Mei Hong, Massachusetts Institute of Technology, USA

Juli Feigon, University of California, Los Angeles, USA
Integrative Structural Biology of RNPs

Remco Sprangers, Regensburg University, Germany
Beyond Static Structures: Linking Enzyme Dynamics with Function

Coffee Break

Teresa Carlomagno, University of Birmingham, UK
High Molecular-Weight Complexes in the Regulation of Gene Expression: A View by Integrative Structural Biology

Guillaume Mas, Biozentrum, Switzerland
Short Talk: Functional Cycle of the Human Hsp70 Chaperone BiP at Atomic Resolution

Qishun Zhou, Medical University of Graz, Austria
Short Talk: Tyrosine Mediated Nuclear Import of CIRBP Reveals a Flexible NLS Recognition by TNPO3

12:00—13:00
Lunch

Ballroom
13:00—15:00
Workshop and Panel Discussion: Is NMR Spectroscopy a Necessary Tool in Structural Biology?

Auditorium
* Teresa Carlomagno, University of Birmingham, UK

Martin Blackledge, Institut de Biologie Structurale, France
Panelist

Mei Hong, Massachusetts Institute of Technology, USA
Panelist

Juli Feigon, University of California, Los Angeles, USA
Panelist

Charalampos Babis Kalodimos, St. Jude Children's Research Hospital, USA
Panelist

Yukti Khanna, Medical University of Graz, Austria
The Disordered Regions of p53 and the mRNA Export Factor ALYREF/THOC4 Regulate the Interaction Between Them

Jobst Liebau, University of Regensburg, Germany
Dynamics and Interactions in the 410 kDa RNA Exosome Complex

Vittoria Nanna, University of Birmigham, UK
Understanding K15-dependent Activation of PLC-γ1 in Kaposi's Sarcoma Herpesvirus Infections

Micael Simões Silva, Weizmann Institute of Science, Israel
Structural Basis of a Marginally Stable Client Recognition by an Hsp40/JDP Chaperone

15:00—15:30
Coffee Available

Foyer
15:30—18:00
Structural Biology of Complex Systems by NMR and Other Methods

Auditorium
* Ichio Shimada, University of Tokyo, Japan

Melinda J. Duer, University of Cambridge, UK
The Chemistry of Ageing and Calcification in the Extracellular Matrix

Lynette Cegelski, Stanford University, USA
EM and ssNMR of Bacterial Extracellular Matrix

Stephan Grzesiek, University of Basel, Switzerland
Protein Allosteric Functional Mechanisms Determined by High-Resolution NMR

Sigrid Milles, FMP, Germany
Studying Intrinsically Disordered Proteins by Integrated NMR and Single Molecule FRET

18:00—19:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.

Ballroom

WEDNESDAY, MARCH 29

08:30—09:00
Poster Setup

Ballroom
09:00—15:00
Poster Viewing

Ballroom
09:00—12:00
Dynamic Structures and Function

Auditorium
* Remco Sprangers, Regensburg University, Germany

Martin Blackledge, Institut de Biologie Structurale, France
NMR Provides Unique Insight into the Role of Conformational Disorder in Viral Function

Rina Rosenzweig, Weizmann Institute of Science, Israel
Molecular Chaperones in Health and Disease - What we can Learn by NMR

Coffee Break

Charalampos Babis Kalodimos, St. Jude Children's Research Hospital, USA
The Conformational Landscape of Protein Kinases in Physiology and Disease

Soichiro Kitazawa, Ritsumeikan Univ., Japan
Short Talk: Amplification of the Specific Conformational Fluctuation of Outer surface protein A by Mutagenesis and Hydrostatic Pressure

Jordan Chill, Bar Ilan University, Israel
Short Talk: The Case of WIP: From Disordered Multitasker to Determinant of Wiskott-Aldrich Syndrome

12:00—13:00
Lunch

Ballroom
13:00—15:00
Poster Session

Ballroom
15:00—18:00
Conformational Changes in Membrane Proteins

Auditorium
* Charalampos Babis Kalodimos, St. Jude Children's Research Hospital, USA

Ichio Shimada, University of Tokyo, Japan
Function-Related Dynamic of Membrane Proteins

Clemens Glaubitz, University of Frankfurt, Germany
Resolving Functional Mechanisms for Membrane Proteins by DNP and High-Resolution Solid-State NMR

Coffee Break

Mei Hong, Massachusetts Institute of Technology, USA
Protein Dynamics and Protein-Ligand Interactions in Lipid Membranes from Solid-State NMR

Josh Wand, Texas A&M University, USA
Short Talk: The Role of Internal Motion and Conformational Entropy in Membrane Protein Stability and Function

Ute Hellmich, Friedrich Schiller University Jena, Germany
Short Talk: Crosstalk between regulatory elements in the disordered TRPV4 N-terminus modulates lipid-dependent channel activity

18:00—19:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.

Ballroom

THURSDAY, MARCH 30

09:00—12:00
Nucleic Acids Structure and Function

Auditorium
* Rina Rosenzweig, Weizmann Institute of Science, Israel

Harald Schwalbe, University of Frankfurt, Germany
Conformational Dynamics Sustains Regulatory Signaling in Riboswitches

Hashim M. Al-Hashimi, Columbia University, USA
Remote Presentation: Revealing the Role of Conformational Penalties in RNA Cellular Activity

Coffee Break

Qi Zhang, University of North Carolina at Chapel Hill, USA
RNA Dynamics Underlines the Functional Mechanism of Riboswitches

Philipp Innig Aguion, University of Birmingham, UK
Short Talk: Methods for Structure Determination of RNA by Solid-State NMR

Sabrina Töws†, Goethe-University, Germany
Short Talk: NMR-based Structural and Functional Characterisation of the SARS-CoV-2 Stem-Loop 1

12:00—13:00
Lunch

Ballroom
13:00—15:00
Methodological Frontiers and Sister Spectroscopies

Auditorium
* Harald Schwalbe, University of Frankfurt, Germany

Haribabu Arthanari, Harvard Medical School, USA
Emerging Methods in Solution NMR to Address Blindspots in Structural Biology

Enrica Bordignon, University of Geneva, Switzerland
Combining Cryo-EM and EPR Helps to Unveil Intermediate States in the Prepore to Pore Transition of a Tc Toxin

Richard A. Stein, Vanderbilt University, USA
Integrated Alphafold2 and DEER Spectroscopy to Illuminate the Conformational Dynamics of Transporters

Geerten W. Vuister, University of Leicester, UK
Short Talk: Robust Automated- and Interactive Protocols for NMR-based Fragment Drug Discovery and Design (FBDD) using the CcpNmr AnalysisScreen Programme

15:00—15:15
Meeting Wrap-Up: Outcomes and Future Directions (Organizers)

Auditorium

FRIDAY, MARCH 31

08:00—08:00
Departure


*Session Chair †Invited, not yet responded.



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