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Boston Park Plaza Floorplan

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This meeting took place in 2013

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Positive Strand RNA Viruses (D7)

Organizer(s) Eric J. Snijder and Ralf Bartenschlager
April 28—May 3, 2013
Boston Park Plaza • Boston, MA USA
Abstract Deadline: Dec 20, 2012
Late Abstract Deadline: Jan 31, 2013
Scholarship Deadline: Dec 20, 2012
Early Registration Deadline: Feb 28, 2013

Supported by the Directors' Fund

Summary of Meeting:
Originally initiated in 1986 as a Keystone Symposia meeting, and later continued by Dr. Margo Brinton as primary organizer for more than 25 years, the triennial international symposium on Positive Strand RNA Viruses has provided an international forum for recent research on human, animal, plant, insect, and bacterial viruses with positive-strand RNA genomes. These pathogens constitute the largest group of viral agents including a large number of important human pathogens like hepatitis C virus, West Nile and dengue viruses, hepatitis A virus and poliovirus. Furthermore, the enormous variety of positive-stranded RNA viruses found in animals, and their evolutionary potential, continues to pose a threat of novel (zoonotic) infections emerging in human populations as illustrated by the emergence of the SARS coronavirus. The 2013 meeting on Positive Strand RNA Viruses, now back under the roof of Keystone Symposia, will cover both basic and applied research and provide an unparalleled platform to discuss the unique and common features of the members of this important virus group. Experienced and junior researchers will come together to discuss the latest developments in the areas of the molecular biology, pathogenesis, evolution and epidemiology of positive-stranded RNA viruses, aiming to translate these findings into novel approaches to diagnose and control infections by these agents.

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No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 24 hr (international) time


4:00—8:00 PM
Arrival and Registration

Imperial Check Room


7:30—8:30 AM

Statler/Imperial Ballroom
8:30—9:30 AM
Welcome and Keynote Address

Imperial Ballroom
Herbert (Skip) W. Virgin, Vir Biotechnology, USA
The Virome and Disease Susceptibility: More Than One Way to Be a Pathogen (Or a Friend)

9:30—11:45 AM
Innate Immunity: Sensing and Defending

Imperial Ballroom
* Frank van Kuppeveld, Utrecht University, Netherlands

Takashi Fujita, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan
Size-Dependent Sensing of Viral RNA during Antiviral Responses

Sun Hur, Harvard Medical School, USA
Short Talk: Molecular Mechanism for Viral dsRNA Detection by MDA5 Filaments

* John W. Schoggins, University of Texas Southwestern Medical Center, USA
Antiviral Type I Interferon Effectors Targeting RNA Viruses

Margo A. Brinton, Georgia State University, USA
Anti-Flaviviral Activities of 2'-5' Oligoadenylate Synthetase 1

10:10—10:30 AM
Coffee Break

Imperial Foyer
11:45 AM—1:00 PM
Poster Setup

Plaza Ballroom
12:00—1:00 PM

Statler/Imperial Ballroom
12:30—10:00 PM
Poster Viewing

Plaza Ballroom
12:30—3:00 PM
Poster Session 1

Plaza Ballroom
3:00—3:30 PM
Coffee Available

Imperial Foyer
3:30—5:30 PM
Evolution of Viruses in the Environment

Imperial Ballroom
* Raul Andino, University of California, San Francisco, USA
Dynamics of the Mutational and Fitness Landscape of RNA Virus Populations

* Marco Vignuzzi, Pasteur Institut, France
Virus Micro-Evolution and Subpopulations within the Infected Host

Alexander E. Gorbalenya, Leiden University Medical Center, Netherlands
Short Talk: Learning about RNA Viruses from their Genome Sequences

Marilyn J. Roossinck, Pennsylvania State University, USA
The Big Unknown: Plant Virus Biodiversity and Ecosystem Impacts

5:30 PM
On Own for Dinner


7:30—8:30 AM

Statler/Imperial Ballroom
8:30 AM—8:00 PM
Poster Viewing

Plaza Ballroom
8:30—11:30 AM
Systems Biology of +RNA Virus-Host Interaction

Imperial Ballroom
* Nihal Altan-Bonnet, NHLBI, National Institutes of Health, USA

* Carolyn B. Coyne, University of Pittsburgh, USA
RNAi Screening to Identify Novel Regulators of Viral Infections

Peter D. Nagy, University of Kentucky, USA
Yeast as a Model Host to Explore Virus - Host Interactions

Ralf Bartenschlager, Heidelberg University, Germany
Host Cell Factors and Pathways Utilized for Flavivirus Replication

Olha Ilnytska, Rutgers State University of New Jersey, USA
Short Talk: Lipid Blueprints for Enteroviral Replication Derive from Endocytic Rewiring of Cellular Cholesterol Landscape

Andrew Varble, Mount Sinai School of Medicine, USA
RNAi Screening in vivo Identifies Critical Components to the Antiviral Response

9:50—10:10 AM
Coffee Break

Imperial Foyer
On Own for Lunch and Recreation

2:00—4:30 PM
Workshop 1

Imperial Ballroom
* Susan C. Baker, Loyola University Chicago, USA

* Eric J. Snijder, Leiden University Medical Center, Netherlands

Marlene Dreux, CIRI; Université de Lyon; Inserm U1111, France
Exosomal Transfer of Viral RNA to Plasmacytoid Dendritic Cells Triggers Innate Immunity

Sonja M. Best, NIAID, National Institutes of Health, USA
The Mechanism of Type I Interferon Antagonism by NS5 from Encephalitic Flaviviruses, TBEV and WNV

Ana Fernandez-Sesma, Icahn School of Medicine at Mount Sinai, USA
Dengue Virus (DENV) Inhibits Type I Interferon (IFN) in Infected Cells via the Cleavage of Human STING by the NS2B3 Viral Protease

Laura D. Kramer, Wadsworth Center, New York State Department of Health; SUNY Albany, USA
Biological Consequences of West Nile Virus Adaptation to a Mosquito Vector: Evolution of Virulence

Yorgo Modis, Yale University, USA
Crystal Structure of Glycoprotein E2 from Bovine Viral Diarrhea Virus

Berend Jan Bosch, Utrecht University, Netherlands
Dipeptidyl Peptidase 4 Is a Functional Receptor for the Emerging Human Coronavirus-EMC

Christopher M. Robinson, Indiana University, USA
A Two-Component Mechanism for Bacterial Polysaccharide-Mediated Enhancement of Poliovirus Infectivity

Caleb D. Marceau, Stanford University, USA
Identification of Host Factors Required for Dengue Virus Pathogenesis Using a Human Haploid Genetic Screen

Ana P. Goncalvez, Sanofi Pasteur, USA
RepliVax-TBE, a Novel Single-Dose Vaccine Against Tick-Borne Encephalitis

Pei-Yong Shi, University of Texas Medical Branch, USA
Flavivirus RNA Methylation for Vaccine Development

4:30—5:00 PM
Coffee Available

Imperial Foyer
5:00—7:00 PM
Entry and Assembly

Imperial Ballroom
* Brett D. Lindenbach, Yale University School of Medicine, USA

* Sara R. Cherry, University of Pennsylvania, USA
Discovering New Players in Alphavirus Entry Using RNAi Screening

William M. Gelbart, University of California, Los Angeles, USA
Assembly of Bromo and Alpha Viruses and their Hybrids

Joyce Jose, Pennsylvania State University, USA
Short Talk: Analysis of Spatial and Temporal Regulation of Alphavirus Replication and Budding in Live Cells Using Fluorescent Dual-Labeled Sindbis Virus

Glenn C. Randall, University of Chicago, USA
Mechanisms of Hepatitis C Virus Assembly and Egress

7:00 PM
On Own for Dinner

8:00—10:00 PM
Poster Session 1 Tear Down

Plaza Ballroom


7:30—8:30 AM

Statler/Imperial Ballroom
7:30—8:30 AM
Poster Setup

Plaza Ballroom
8:30 AM—10:00 PM
Poster Viewing

Plaza Ballroom
8:30—11:30 AM
Adaptive Immunity and Vaccines

Imperial Ballroom
* Diane E. Griffin, Johns Hopkins Bloomberg School of Public Health, USA
Alphavirus Encephalitis: Mechanisms of Virus Clearance

Robert Thimme, University Medical Center Freiburg, Germany
Failure of Adaptive Immunity to Clear HCV Infection

Swati Mukherjee, NIAID, National Institutes of Health, USA
Short Talk: Exploring the Mechanism and Significance of Cell Type-Dependent Neutralization of Flaviviruses

* Christian W. Mandl, Novartis Vaccines and Diagnostics, Inc., USA
Alphavirus-Vectored Vaccines: From VRP to SAM® Vaccines

Kim Y. Green, NIAID, National Institutes of Health, USA
Norovirus Vaccines and Immunity

9:50—10:10 AM
Coffee Break

Imperial Foyer
11:30 AM—12:30 PM

Statler/Imperial Ballroom
12:00—2:30 PM
Poster Session 2

Plaza Ballroom
2:30—3:00 PM
Coffee Available

Imperial Foyer
3:00—5:00 PM
RNA Structure and Function

Imperial Ballroom
* Peter D. Nagy, University of Kentucky, USA

* Andrea Gamarnik, Fundación Instituto Leloir, Argentina
Dengue Virus Genome Plasticity and Host-Specific Functions of Viral RNA Structures

Peter Sarnow, Stanford University School of Medicine, USA
MicroRNAs and their Roles in Regulating Hepatitis C Virus Replication

Ian Brierley, University of Cambridge, UK
Translational Gymnastics of RNA Viruses

Ying-Han Chen, Rutgers University, USA
Short Talk: Divide and Conquer: Spatial Segregation of Viral RNA Translation and Synthesis Revealed by Super-Resolution Imaging

5:00—5:45 PM
Workshop: Virus Pathogen Resource (ViPR)
ViPR is a freely available, web-based NIAID Bioinformatics Resource Center supporting human viral pathogen research. ViPR captures various types of information including sequence records, gene and protein annotations, 3D protein structures, immune epitope locations, clinical and surveillance data, host factor data, and novel data derived from comparative genomics analysis. Analytical and visualization tools for metadata-driven statistical sequence analysis, multiple sequence alignment, phylogenetic tree construction, sequence variation determination, genome annotation, genotype recombination detection, PCR primer design, and 3D protein structure visualization are also provided. This workshop will provide a live demonstration of ViPR's major search and analysis capabilities.

Imperial Ballroom
* Yun Zhang, J. Craig Venter Institute, USA

5:45 PM
On Own for Dinner

Plaza Ballroom


7:30—8:30 AM

Statler/Imperial Ballroom
8:30—11:30 AM
Replication Enzymes and their Inhibition

Imperial Ballroom
* Alexander E. Gorbalenya, Leiden University Medical Center, Netherlands

* Bruno Canard, CNRS and University of Marseille, France
The Interplay of Viral RNA Synthesis, Repair, and Capping in (+)RNA Viruses

Eric J. Snijder, Leiden University Medical Center, Netherlands
Arterivirus NSP2: Versatility at the RNA and Protein Level

Núria Verdaguer, Institut de Biologia Molecular de Barcelona (CSIC), Spain
Foot and Mouth Disease Virus RNA-Dependent RNA Polymerase: Structure and Fidelity

David J. Barton, University of Colorado Denver, USA
Short Talk: Picornavirus Polymerase Structures Involved in the Polyadenylation of Viral RNA

John O. Link, Gilead Sciences Inc., USA
Inhibitors of Hepatitis C Virus Replication: Viral and Host Cell Targets

9:50—10:10 AM
Coffee Break

Imperial Foyer
1:30—2:00 PM
Poster Session 2 Tear Down

Plaza Ballroom
On Own for Lunch and Recreation

2:00—4:30 PM
Workshop 2

Imperial Ballroom
* Margaret R. MacDonald, Rockefeller University, USA

* Ralf Bartenschlager, Heidelberg University, Germany

Gerald M. McInerney, Karolinska Institutet, Sweden
Sequestration of G3BP by Semliki Forest Virus nsP3 Inhibits the Assembly of Stress Granules on Viral mRNAs

Kristiina Maria Mäkinen, University of Helsinki, Finland
Ribosomal Protein P0 in Potato Virus A Infection

Isabel Sola, Centro Nacional Biotecnología (CNB-CSIC), Spain
High-Order Structures in the Coronavirus Genome Mediated by a Novel Long Distance RNA-RNA Interaction Promote Discontinuous RNA Synthesis during Transcription

Feng Yang, Yale School of Medicine, USA
Trans-Complementation of Hepatitis C Virus Nonstructural Genes Reveals Unusual Cis-Requirements for NS5A and NS5B

Tero Ahola, University of Helsinki, Finland
The Roles of Template RNA and Replication Proteins in the Formation of Semliki Forest Virus Replication Spherules

George A. Belov, University of Maryland, USA
Activation of Fatty Acid Import Triggers Development of Picornavirus Replication Organelles

Hilde van der Schaar, Utrecht University, Netherlands
A Novel, Broad-Spectrum Inhibitor of Enterovirus Replication that Targets Host Cell Factor PI4KIIIbeta

Lara J. Herrero, Griffith University, Australia
A Critical Role for Macrophage Migration Inhibitory Factor (MIF) in Alphavirus Virus Induced Inflammation, Arthritis and Myositis

Stephen Berryman, Pirbright Institute, UK
Foot-and-Mouth Disease Virus Induces Autophagosomes during Cell Entry via a Class III Phosphatidylinositol 3-Kinase-Independent Pathway

L. Dillon Birdwell†, University of Pennsylvania, USA
Activation of the OAS–RNase L Pathway by Murine Coronavirus Is Cell- and Organ-Type Specific

4:30—5:00 PM
Coffee Available

Imperial Foyer
5:00—7:00 PM
Pathogenesis (Includes UPR, Stress Responses, Autophagy)

Imperial Ballroom
* Susan R. Weiss, University of Pennsylvania, USA

Jari P.T. Valkonen, University of Helsinki, Finland
Plant Potyvirus-Host Interactions: HCpro and VPg

Matthew Frieman, University of Maryland, USA
Short Talk: Induction of Alternatively Activated Macrophages Enhances Pathogenesis during SARS Coronavirus Infection

* Stanley M. Lemon, University of North Carolina at Chapel Hill, USA
Membrane Hijacking: Biogenesis of Enveloped Hepatoviruses and Role in Pathogenesis of Hepatitis A

Ann Palmenberg, University of Wisconsin-Madison, USA
Comparative Structure and Anti-Host Activities of 2A Proteases from Rhinovirus A, B and C

7:00—8:00 PM
Social Hour with Lite Bites

Plaza Ballroom
8:00—11:00 PM

Plaza Ballroom



*Session Chair †Invited, not yet responded.

We gratefully acknowledge support for this conference from:

Directors' Fund

These generous unrestricted gifts allow our Directors to schedule meetings in a wide variety of important areas, many of which are in the early stages of research.

Click here to view all of the donors who support the Directors' Fund.

We gratefully acknowledge the generous grant for this conference provided by:

National Institutes of Health

Grant No. 1R13AI106102-01

The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention of trade names, commercial practices, or organizations imply endorsement by the U.S. Government.

We gratefully acknowledge additional support for this conference from:

International Symposium on Positive Strand RNA Viruses

We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:

Click here to view more of these organizations

Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:

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If your organization is interested in joining these entities in support of Keystone Symposia, please contact: Sarah Lavicka, Director of Corporate Relations, Email:,
Phone:+1 970-262-2690

Click here for more information on Industry Support and Recognition Opportunities.

If you are interested in becoming an advertising/marketing in-kind partner, please contact:
Nick Dua, Senior Director, Communications, Email:,
Phone:+1 970-262-1179