Keystone Symposia

Hilton Santa Fe Historic Plaza Hotel Floorplan

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This meeting took place in 2005



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Plant Cell Signaling: In vivo and omics Approaches (B1)


Organizer(s) Sarah M. Assmann and Alan M. Jones
February 1—6, 2005
Hilton Santa Fe Historic Plaza Hotel • Santa Fe, NM USA
Abstract Deadline: Sep 30, 2004
Late Abstract Deadline:
Scholarship Deadline:
Early Registration Deadline: Dec 1, 2004

Supported by The Director's Fund

Summary of Meeting:
Much of the most exciting current research on cell signaling involves in vivo and large scale ("omic") techniques. In vivo techniques, especially those utilizing advanced GFP-based imaging methods, are being brought to bear on studies of signal transduction in "real time" and "in planta," thus allowing never-before-seen glimpses into the functioning of living plant cells. At the same time, "omic" techniques are providing us with overviews of the entire function of a cell or tissue at the levels of the genome, the transcriptome, the proteome, and the metabolome. This meeting will bring together leaders in cell signaling research, spanning the gamut from cell biologists who observe single cells, to genomicists uncovering whole-genome views of transcription, to mathematicians who seek to build testable models of cellular processes. Key signaling elements, including G proteins, hormonal signaling, and dynamic organization of the nucleus and cytoskeleton are some of the biological processes that are highlighted. Presentation of cutting-edge data and techniques, including a workshop on FRET methodology, and the Keystone Symposium format itself will promote discussion and collaboration among participants.

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No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 24 hr (international) time


TUESDAY, FEBRUARY 1

3:00—7:30 PM
Registration

Promenade
6:30—7:30 PM
Welcome Refreshments

Promenade
7:30—8:30 PM
Keynote Address

Mesa A-B
Michael P. Snyder, Stanford University School of Medicine, USA
Analyze This and That: Genomes and Proteomes


WEDNESDAY, FEBRUARY 2

7:00—8:00 AM
Breakfast

Chamisa
8:00—11:00 AM
Hormones and Signal Transduction

Mesa A-B
Tatsuo Kakimoto, Osaka University, Japan
Cytokinin Signaling

Randy D. Allen, Oklahoma State University, USA
Short Talk: Brassinosteroid Signaling in Cotton Fiber Development

Frank L.H. Menke, Sainsbury Laboratory, UK
Short Talk: Phosphoproteomics of JA-Dependent Induced Systemic Rresistance

Jutta Ludwig-Müller, Technische Universitaet Dresden, Germany
Short Talk: Transcriptome Analysis of the Clubroot Infection of Arabidopsis thaliana Revealed Novel Roles for Plant Hormones as Signals during Root Gall Development

Claus Schwechheimer, Technische Universitat Munchen, Germany
Short Talk: Control of Plant Development by Kinases Interacting with DELLA-Domain Proteins.

Hak Soo Seo, Seoul National University, South Korea
Interaction of Sumoylation and Ubiquitination in Plant Cell Signaling

* Mark A. Estelle, University of California, San Diego, USA
Protein Turnover in Auxin Signaling

9:20—9:40 AM
Coffee Break

Promenade
11:00 AM—1:00 PM
Poster Setup

Ortiz
1:00—10:00 PM
Poster Viewing

Ortiz
4:30—4:45 PM
Coffee & Snacks Available

Promenade
4:45—7:00 PM
Visualizing Molecular Activities in vivo

Mesa A-B
Jennifer A. Lippincott-Schwartz, Howard Hughes Medical Institute - Janelia Farm, USA
Photoactivatable GFPs and Intracellular Protein Dynamics

Klaus M. Hahn, University of North Carolina at Chapel Hill, USA
Novel dyes enable sensitive imaging of endogenous protein conformational changes in living cells - Cdc42 and Erk2

* Dorus Gadella, University of Amsterdam, Netherlands
Visualizing Cytoskeletal Dynamics and Cellular Signaling with GFP-Based Multimode Fluorescence Microscopy

Robyn M. Perrin, University of Wisconsin-Madison, USA
Short Talk: WVD2, a Novel Microtubule Binding Protein Affecting Plant Anisotropic Cell Expansion, Morphology and Helical Handedness

7:00—8:00 PM
Social Hour

Chamisa
7:30—10:00 PM
Poster Session 1

Ortiz

THURSDAY, FEBRUARY 3

7:00—8:00 AM
Breakfast

Chamisa
8:00—11:15 AM
G Protein Regulation of Cellular Processes

Mesa A-B
* Sarah M. Assmann, Pennsylvania State University, USA
Heterotrimeric G Protein Signaling in Arabidopsis ABA Responses

Alan M. Jones, University of North Carolina at Chapel Hill, USA
Sugar Sensing in Plants Coupled by a Heterotrimeric G Protein

Tian Jin, NIAID, National Institutes of Health, USA
Visualization of G-Protein Localization and Activity during Dictyostelium Chemotaxis

Ralph Panstruga, Max-Planck-Institut für Züchtungsforschung, Germany
Serpentine Plant MLO Proteins as Entry Portals for Powdery Mildew Fungi

Ko Shimamoto, Nara Institute of Science and Technology, Japan
G Proteins and Disease Resistance in Rice

9:20—9:40 AM
Coffee Break

Promenade
11:15 AM—12:00 PM
Poster Setup

Ortiz
11:15 AM—12:00 PM
Lunch

Chamisa
12:00—2:00 PM
Workshop: FRET Techniques

Mesa A-B
* Alan M. Jones, University of North Carolina at Chapel Hill, USA

J. Philip Taylor, University of North Carolina at Chapel Hill, USA
FRET Analysis in Plant Cells: Trials and Tribulations

Tian Jin, NIAID, National Institutes of Health, USA
Spectrally Resolved FRET Time-Lapse Imaging Detects Dynamic Protein-Protein Interactions in Single Living Cells

Simon Gilroy, University of Wisconsin, USA
Imaging Ionic Signaling in the Arabidopsis Root

Raymond Zielinski, University of Illinois at Urbana-Champaign, USA
Fluorescent Reporters of Downstream Events in Ca2+ Signaling Pathways

Albrecht G. Von Arnim, University of Tennessee, USA
In vivo Protein-Interaction Assays Based on Bioluminescence Resonance Energy Transfer (BRET)

Karin Schumacher, University of Tuebingen, Germany
Bimolecular Fluorescence Complementation (BiFC) in Plant Cells

2:00—4:30 PM
Demonstration of FRET Microscope Platforms and Software by Olympus and Scanalytics

Ortiz
2:00—4:30 PM
Poster Session 2

Ortiz
4:30—4:45 PM
Coffee & Snacks Available

Promenade
4:45—7:00 PM
Intracellular Signaling I

Mesa A-B
Federica Brandizzi, Michigan State University, USA
In vivo Dynamics of the Plant Endomembrane System

Ying Gu, , USA
Short Talk: ROP GTPases and Cytoskeletal Dynamics in Pollen Tubes

* Natasha V. Raikhel, University of California, Riverside, USA
The Power of Chemical Genomics to Discover Proteins that Mediate Endomembrane Trafficking in Plants

Frederick M. Ausubel, Massachusetts General Hospital, USA
PAMP-Elicited Innate Immune Signaling Pathways in Arabidopsis

7:00—10:00 PM
Poster Viewing or Free for Dinner

Ortiz

FRIDAY, FEBRUARY 4

7:00—8:00 AM
Breakfast

Chamisa
8:00—11:00 AM
Intracellular Signaling II

Mesa A-B
Gareth H. Jones, University of Birmingham, UK
The Control of Meiotic Recombination: A Cytological Perspective

* Gregory P. Copenhaver, University of North Carolina at Chapel Hill, USA
A New View of Meiotic Recombination

Joanne Chory, The Salk Institute, USA
Plastid to Nucleus Signaling

Maureen R. Hanson, Cornell University, USA
Short Talk: Intracellular Communication Through Stromules, Mobile Tubular Plastid Projections

Masamitsu Wada, Kyushu University, Japan
Light-Induced Chloroplast Relocation Movement

9:20—9:40 AM
Coffee Break

Promenade
11:00 AM—1:00 PM
Poster Setup

Ortiz
1:00—10:00 PM
Poster Viewing

Ortiz
4:30—4:45 PM
Coffee & Snacks Available

Promenade
4:45—7:00 PM
Single Cells and New Techniques

Mesa A-B
Wolf B. Frommer, Carnegie Institution for Science, USA
Metabolite Imaging with FRET-Based Nanosensors

* Simon Gilroy, University of Wisconsin, USA
Ca2+ and pH Integrate Tropic Signaling in the Root

Soren Friis, Royal Veterinary and Agricultural University, Denmark
Short Talk: Novel GFP Based pH-Sensors for Monitoring H+-ATPase Activity in Plant Root Hair Cells of Arabidopsis thaliana

Helen E. Blackwell, University of Wisconsin-Madison, USA
Small Molecule Control of Plant/Microbe Interactions

Patrick S. Schnable, Iowa State University, USA
Laser Capture Microdissection and Cell-Type-Specific Gene Expression

7:00—8:00 PM
Social Hour

Chamisa
7:30—10:00 PM
Poster Session 3

Ortiz

SATURDAY, FEBRUARY 5

7:00—8:00 AM
Breakfast

Chamisa
8:00—11:00 AM
Cellomics I

Mesa A-B
* Julian I. Schroeder, University of California, San Diego, USA
Guard Cell Signal Transduction from Genomics to Cellular Signaling Dynamics

Bernhard O. Palsson, University of California, San Diego, USA
Bringing Genomes to Life: The Use of Genome-Scale in Silico Models

Waltraud Schulze, University of Southern Denmark, Denmark
Short Talk: Proteomic Strategies to Analyze Modification-Dependent Protein-Protein Interactions

Daphne Preuss, Chromatin Inc., USA
Proteomics: Pollen Coat Proteins and Ecotypic Variation

Marcus Heisler, EMBL Heidelberg, Germany
The Computable Plant

9:20—9:40 AM
Coffee Break

Promenade
4:30—4:45 PM
Coffee & Snacks Available

Promenade
4:45—7:00 PM
Cellomics II

Mesa A-B
* Joseph R. Ecker, The Salk Institute for Biological Studies, USA
Genome-Wide Discovery of Transcription Units and Functional Elements in Arabidopsis

Mark Stitt, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Germany
Transcriptomics, 'Nzynomics and Metabolomics of Carbon and Nitrogen Assimilation

Pierre Broun, University of York, UK
Short Talk: A Genomic Investigation of Metabolic Regulation in Tomato Trichome Secretory Cells

Scott C. Peck, University of Missouri, USA
Phosphoproteomics of Plant-Microbe Interactions: New Components, New Levels of Complexity

6:45—7:00 PM
The Future

Mesa A-B
Joseph R. Ecker, The Salk Institute for Biological Studies, USA

Daphne Preuss, Chromatin Inc., USA

7:00—8:00 PM
Social Hour

Mesa C
8:00—11:00 PM
Entertainment

Mesa C

SUNDAY, FEBRUARY 6

 
Departure


*Session Chair †Invited, not yet responded.



We gratefully acknowledge support for this conference from:


Directors' Fund


These generous unrestricted gifts allow our Directors to schedule meetings in a wide variety of important areas, many of which are in the early stages of research.

Click here to view all of the donors who support the Directors' Fund.



We gratefully acknowledge additional support for this conference from:

Pioneer Hi-Bred a DuPont business

We appreciate the organizations that provide Keystone Symposia with additional support, such as marketing and advertising:


Click here to view more of these organizations


Special thanks to the following for their support of Keystone Symposia initiatives to increase participation at this meeting by scientists from underrepresented backgrounds:


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If your organization is interested in joining these entities in support of Keystone Symposia, please contact: Sarah Lavicka, Director of Corporate Relations, Email: sarahl@keystonesymposia.org,
Phone:+1 970-262-2690

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Phone:+1 970-262-1179