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This meeting took place in 2008

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DNA Replication and Recombination (B5)

Organizer(s) Jack D. Griffith, Nancy G. Nossal (in Memoriam), Richard D. Kolodner, Charles S. McHenry and Jerard Hurwitz
February 10—15, 2008
Eldorado Hotel & Spa • Santa Fe, NM USA
Abstract Deadline: Oct 10, 2007
Late Abstract Deadline: Nov 8, 2007
Scholarship Deadline: Oct 10, 2007
Early Registration Deadline: Dec 11, 2007

Supported by the Director's Fund

Summary of Meeting:
The Keystone Symposia “DNA Replication and Recombination” meeting, the longest standing meeting of this multi-decade series continues to meld investigators representing a range of disciplines who otherwise would not have a format for sharing novel approaches and groundbreaking discoveries. This field drives DNA sequencing technology, the design of myriad anti-viral and anti-bacterial drugs, and the development of novel strategies for gene targeting and generation of transgenic animals. Major pivotal questions include (1) the role of condensins in DNA pairing and replication fork movement, (2) mechanisms by which stalled replication forks are recognized and replication is restarted, (3) the role of recombinational mechanisms in the maintenance of telomeres in the absence of telomerase, (4) the architecture of forks and replication origins, and (5) the mechanisms by which fork initiation is timed with the cell cycle. Speakers representing established laboratories will share the podium with young investigators describing genomic, structural, and biochemical approaches to these central questions.

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No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 12 hr (am/pm) time



Zia Concourse

Brief Introduction to the Meeting

* Jack D. Griffith, University of North Carolina, Chapel Hill, USA

Keynote Address

Anasazi Ballroom
Michael E. O'Donnell, Rockefeller University, USA
Dynamic Mechanisms at the E. coli Replication Fork



Eldorado Court
Replication Fork Mechanisms

Anasazi Ballroom
* Peter M. Burgers, Washington University School of Medicine, USA
Remodeling of the Replication Fork in Response to DNA Damage

Thomas A. Steitz, Howard Hughes Medical Institute, Yale University, USA
The Macromolecular Replication Machinery: Structural Insights into the Replisome

Charles S. McHenry, University of Colorado Boulder, USA
Reconstitution of a B. subtilis Rolling Circle DNA Replication System

Antoine M. van Oijen, University of Wollongong, Australia
Under the Hood of the Replisome: A Single-Molecule View of DNA Replication

Coffee Break

Poster Setup

On Own for Lunch

Poster Viewing

Workshop 1: Origins and Okazaki Fragments

Anasazi Ballroom
* Nicholas E. Dixon, University of Wollongong, Australia
A Molecular Mousetrap Determines Polarity in Termination of E. coli DNA Replication

Alan C. Leonard, Florida Institute of Technology, USA
Two Growth Rate-Dependent Mechanisms for Pre-Replication Complex Assembly in E. coli

Kenji Keyamura, Kyushu University, Japan
A Novel Initiation-Stimulator DiaA Directly Promotes ATP-DnaA-Specific Initiation Complexes in E. coli

Smita S. Patel, UMDNJ-RW Johnson Medical School, USA
Coordination of Helicase, Primase, and Polymerase Activities in DNA Replication

Samir M. Hamdan, King Abdullah University of Science and Technology, Saudi Arabia
Two Molecular Triggers Reset the Bacteriophage T7 Replisome after Okazaki Fragment Synthesis

Isabel Kurth, Rockefeller University, USA
Polymerase Collision Release is Mediated by Loss of Multiple Attachment Points to DNA upon Completing each Okazaki Fragment

Erik Johansson, Umeå University, Sweden
The Eukaryotic Leading and Lagging Strand DNA Polymerases are Loaded onto Primer-Ends via Separate Mechanisms but have Comparable Processivity in the Presence of PCNA

Nina Y. Yao, Rockefeller University, USA
Mechanism of PCNA Clamp Opening by Replication Factor C

Workshop 2: Chromosomes, Telomeres, and Large Scale Organization

Zia Ballroom
* David J. Sherratt, University of Oxford, UK
Independent positioning and action of replication forks in live Esherichia coli

Kiyoshi Mizuuchi, NIDDK, National Institutes of Health, USA
P1 Plasmid Partition: Role of ATP-Dependent ParA Conformational Change and DNA Binding Kinetics in DNA Translocation

Shingo Nozaki, Nagoya University Graduate School, Japan
Subcellular Localization of Escherichia coli DnaA, the Initiator of DNA Replication

Bhupender Singh, Uppsala University, Sweden
Replication Factories in Live Bacteria (E. coli): Localization for Chromosomal, Viral and Plasmid Replication Sites

Amitabh Nimonkar, University of California, USA
Single-Molecule Imaging & Biochemical Analysis of Saccharomyces Cerevisiae Encoded Dmc1 & Tid1/Rdh54 Proteins

Constance Nugent, University of California, Riverside, USA
Analysis of Telomere Capping Protein Interactions with Polalpha Subunits

Toru Nakamura, University of Illinois at Chicago, USA
Cell Cycle Regulation of Fission Yeast Telomere Proteins

Manolis Papamichos-Chronakis, Institut Curie, France
The INO80 Chromatin Remodeling Enzyme Regulates Replisome Function and Stability

Coffee Available

Replication and Recombination at Telomeres

Anasazi Ballroom
* Jack D. Griffith, University of North Carolina, Chapel Hill, USA
T-Loops and T-Circles in the Replication of Telomeres

Julia Promisel Cooper, NCI, National Institutes of Health, USA
Disentangling Chromosome Ends: The Outcome of Replication Fork Stalling at Telomeres is Dictated by Sumoylation of RecQ Helicase

Susan M. Bailey, Colorado State University, USA
Telomere Dynamics and Premature Aging

Social Hour with Lite Bites

Poster Session 1




Eldorado Court
Poster Setup

Assembling Initiation Complexes - Replication Origins and Cell Cycle Control

Anasazi Ballroom
* Jerard Hurwitz, Memorial Sloan-Kettering Cancer Center, USA
Initiation of Replication

Louise T. Chow, University of Alabama at Birmingham, USA
Regulation of Human Papillomavirus DNA Replication

Bruce W. Stillman, Cold Spring Harbor Laboratory, USA
Control of the Chromosome Cycle in Human Cells by the Origin Recognition Complex

John F.X. Diffley, Francis Crick Institute, UK
Regulation of Replication Origin Activation during the Cell Cycle and in Response to DNA Damage

Coffee Break


Poster Session 2

Workshop 3: Helicases, Mitochondrial Replication

Anasazi Ballroom
* Laurie S. Kaguni, Michigan State University, USA
Modular Architecture and Human Disease Alleles of the Mitochondrial Replicative DNA Helicase

Amy Marion Branagan, University of Vermont, USA
Intermediates in Gp59-Mediated Helicase Loading at the T4 DNA Replication Fork

Ajit Kumar Satapathy, Harvard Medical School, USA
Nucleotide and Metal Cofactor Specificity in Bacteriophage T7 Helicase

Whitney Yin, University of Texas, Austin, USA
Crystal Structure of Human Miochondrial DNA Polymerase Holoenzyme

Robert A. Sclafani, University of Colorado Denver, USA
Structure-Function Analysis of DNA Binding Domains in Mcm DNA Helicases

Matthew L. Bochman, Indiana University, USA
Involvement of an ATP-Dependent “Gate” in MCM2-7 Helicase Activity

Megan Davey, University of Western Ontario, Canada
A Ringmaker Mechanism for Loading MCMs onto DNA

Jaya G. Yodh, University of Illinois at Urbana-Champaign, USA
Single Molecule Analysis of DNA Unwinding by Human Bloom Helicase

Workshop 4: Recombination and Repair in Yeast and Prokaryotes

Zia Ballroom
* Johannes C. Walter, Harvard Medical School, USA

Chrystelle Maric Antoinat, Institut Andre Lwoff, France
X-Shaped Post-Replicative Joint DNA Molecules Contain Ribonucleotides

Francesca Storici, Georgia Institute of Technology, USA
RNA-Templated DNA Repair

Benedicte Michel, CNRS, France
Ruva Mutants that Resolve Holliday Junctions but do not Revert Replication Forks

Kent W. Mouw, Dana-Farber Cancer Institute, USA
Architecture of a Serine Recombinase – DNA Regulatory Complex

Yasuto Murayama, Yokohama City University, Japan
Fission Yeast Rhp51 Recombinase Promotes DNA Four-Strand Exchange Reaction in vitro

Sapna Das-Bradoo, Northeastern State University, USA
How do Cells Sense DNA Damage “Behind” the Fork?

Kristina Trenz, London Research Institute, Clare Hall, UK
Plx1 is Required for Chromosomal DNA Replication under Stressful Conditions

Audrey J. Klingele, Proteovista LLC, USA
Changes in Gene Expression Related to Acquired Resistance to Radiation Damage

Coffee Available

Recombination Mechanisms and Chromosome Pairing

Anasazi Ballroom
* Kirill S. Lobachev, Georgia Institute of Technology, USA
Mechanisms of Chromosomal Fragility and Genome Rearrangements Mediated by Unstable Repeats

Eric C. Greene, Columbia University, USA
Visualizing DNA Repair Proteins in Action

Camilla Sjögren, Karolinska Institutet, Sweden
Sister Chromatid Cohesion in Chromosome Segregation and Repair

On Own for Dinner



Eldorado Court
Architecture of Replication and Recombination Complexes

Anasazi Ballroom
* Stephen C. West, Francis Crick Institute, UK
Aprataxin — A Proofreader for Abortive DNA Ligation Events

Johannes C. Walter, Harvard Medical School, USA
Mechanism of DNA Interstrand Crosslink Repair

Fred Dyda, NIDDK, National Institutes of Health, USA
Molecular Mechanism of Asymmetric DNA Transposition

James M. Berger, Johns Hopkins University School of Medicine, USA
Molecular Mechanisms for Initiating DNA Replication

Coffee Break

Poster Setup

On Own for Lunch

Poster Viewing

Workshop 5: New Polymerases

Anasazi Ballroom
* Kyungjae Myung, Institute for Basic Science, South Korea

Elena Curti, NICHD, National Institutes of Health, USA
Interplay of E. coli Polymerases during Genome Duplication

Anne-Marie MacLean McRobbie, University of St Andrews, UK
Understanding the Interplay between Recombinases, Helicases and DNA Binding Proteins during Homologous Recombination in Archaea

Danielle L. Daee, , USA
Functional Consequences of Replicative Polymerase Polymorphisms and Cancer-Associated Mutations Modeled in Yeast

Daniel Kane, Tufts University, USA
Usage of DNA Polymerase Eta and Zeta in Homologous Recombination and Repair following DNA Damage in Drosophila

Mitch McVey, Tufts University, USA
DNA Polymerase Theta Functions in an Alternative Double-Strand Break Repair Pathway in Drosophila Melanogaster

Nilabja Sikdar, National Human Genome Research Institute, USA
Human ELG1 Functions to Repair DNA Damage through its Interaction with PCNA and Polymerase eta

Tina M. Albertson, University of Washington, USA
Polymerase Delta and Epsilon Proofreading Act in Parallel Pathways to Suppress Spontaneous Mutation and Cancer in Mice

Garry Dallmann, Venria Bioscience, USA
Identification of Small–Molecule Inhibitors of DNA Polymerase III Holoenzyme

Workshop 6: Recombination and Repair in Higher Eukaryotes

Zia Ballroom
* Richard A. Fishel, Ohio State University, USA

Guo-Min Li, University of Kentucky, USA
Identification of a Novel Mismatch Repair Stimulating Factor involving RFX

Ling Xia, Molecular Medicine and Genetics, USA
The Role of Mismatch Repair and Recombination in the Processing of DNA Alkylating Damage in Living Yeast Cells

Martin Kupiec, Tel Aviv University, Israel
Elg1 is Required for Genome Stability

Renee D. Paulsen, Stanford University, USA
siRNA Screening for Novel Regulators of Genomic Integrity

Kirstin Bass, Mount Sinai School of Medicine, USA
Brc1: Coordinating DNA Repair and Replication

Bernard S. Lopez†, Institut Gustave Roussy, France
PKBalpha/AKT1 Inhibits Homologous Recombination by Cytoplasmic Retention of BRCA1 and Rad51, Mediated by FOXO1a: Impact in Sporadic Breast Cancer

Mark A. Brenneman, Rutgers University, USA
DSS1, a BRCA2-Associated Protein, Regulates Homologous Recombinational Repair in Human Cells

Sandra K. Weller, University of Connecticut School of Medicine, USA
HSV-1 inactivates the ATR pathway during infection by sequestration of ATRIP

Coffee Available

Replication Response to Checkpoints

Anasazi Ballroom
* Stephen P. Bell, Massachusetts Institute of Technology, USA
DDK Targets Mcm 2-7 Proteins in the Pre-RC

Marco F. Foiani, Instituto FIRC di Oncologia Molecolare, Italy
Pathways Controlling Replication Fork Stability

Karlene A. Cimprich, Stanford University, USA
Mechanisms for Maintaining Genome Stability at the Replication Fork

Social Hour with Lite Bites

Poster Session 3




Eldorado Court
Genome Stability and DNA Repair, Alternative Polymerases, DSB Repair

Anasazi Ballroom
* Richard D. Kolodner, Ludwig Institute for Cancer Research, USA
Identification of Pathways that Prevent Genome Instability

Lorena S. Beese, Duke University Medical Center, USA
Human mutS alpha DNA Lesion Recognition Complex

Roger Woodgate, National Institutes of Health, USA
Y-Family Polymerases and Their Role in the Translesion Synthesis of Alkylation Damage

Jan Karlseder, The Salk Institute for Biological Studies, USA
C. elegans Telomeres Contain both G-Strand and C-Strand Overhangs that are Bound by Distinct Telomere-Binding Proteins

Coffee Break

On Own for Lunch

Workshop 7: Fork Stalling And Structure of Fork Complexes

Anasazi Ballroom
* Xiaojiang S. Chen, University of Southern California, USA
The Hexameric Structure of a DnaB-Family Replicative Helicase and its Functional Interplay with Primase

Sharmistha Ghosh, Harvard Medical School, USA
Escherichia Coli Thioredoxin Interacts wiht the Thioredoxin Binding Domain of Bacteriophage T7 DNA Polymerase and with Helicase/Primase at the Replication Fork

Iben Bach Bentsen, Aarhus University, Denmark
Identification of Protein Factors Required during Stalled Replication at a Protein-DNA Barrier

Hong Yan, Fox Chase Cancer Center, USA
Biochemical Analysis of Replication Fork Stall and Restart at a Defined Lesion in Xenopus Nucleoplasmic Extracts

Chiara Indiani, Rockefeller University, USA
Programmed Slowing of the Replication Fork in Response to DNA Damage

Kieran Emptage, University of Newcastle, UK
Archaeal Family B DNA Polymerase Stalling, Protection and Interaction with PCNA at Deaminated Bases

Yu-Hao Paul Chang, University of Southern California, USA
Crystal Structure of the GINS Complex and Functional Insights into its Role in DNA Replication

Roxana Georgescu, Rockefeller University, USA
Structure of a Sliding Clamp on DNA

Workshop 8: VDJ, End Joining and Transposition

Zia Ballroom
* Ronald Chalmers, University of Nottingham Medical School, UK
Base-Flipping Dynamics in V(D)J Recombination

Michael G. Chandler, CNRS UMR 5100, France
Transposition of the Novel Insertion Sequence IS608: Biochemistry and Assimilation into the Cell Cycle

Hiroshi Nakai, Georgetown University Medical Center, USA
Effect of Translation Factor IF2-2 at the Interface of Recombination and Replication for Bacteriophage MU Transposition

Patrick C. Swanson, Creighton University, USA
Evidence for Long Range RAG1/2 Interactions with Coding Sequences in Discrete V(D)J Initiation Complexes Assembled on Coupled Cleavage Substrates

Adam G. W. Matthews, Massachusetts Institute of Technology, USA
RAG2 PHD Finger Couples Histone H3 Lysine 4 Trimethylation with V(D)J Recombination

Lisa Postow, Rockefeller University, USA
Ubiquitin-Mediated Removal of Ku80 from DNA Breaks

John R. Lydeard, Biogen Idec, USA
Break-Induced Replication Requires both the Leading and Lagging Strand Replication Machinery

Jennifer A. Cobb, University of Calgary, Canada
The Mre11 Complex and S Phase: Maintaining the Replisome during Fork Stalling

Coffee Available

Replication Stalling and Restart

Anasazi Ballroom
* Kenneth J. Marians, Memorial Sloan Kettering Cancer Center, USA
Mechanisms of Replication Fork Reactivation

Karim Labib, University of Dundee, UK
Regulating the Progression of Eukaryotic DNA Replication Forks

James E. Haber, Brandeis University, USA
Break Induced-Replication and the Recombination Execution Checkpoint

Social Hour with Lite Bites





*Session Chair †Invited, not yet responded.

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