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This meeting took place in 2016
Here are the related meetings in 2020:
Charting a New Course for Heart Failure: From Discovery to Data (Q8)
For a complete list of the meetings for the upcoming/current season, see our meeting list, or search for a meeting.
Heart Failure: Genetics, Genomics and Epigenetics (Z1)
Organizer(s) Stuart A. Cook, Christine E. Seidman and Yigal M. Pinto
April 3—7, 2016
Snowbird Resort • Snowbird, UT USA
Discounted Abstract Deadline: Dec 3, 2015
Abstract Deadline: Jan 7, 2016
Scholarship Deadline: Dec 3, 2015
Discounted Registration Deadline: Feb 4, 2016
Sponsored by Bayer HealthCare Pharmaceuticals, Pfizer Inc. and Takeda Pharmaceutical Company Limited
Joint Meeting:
Cardiac Development, Regeneration and Repair (Z2)
Summary of Meeting:
Heart failure (HF) is a worldwide epidemic with treatments costing tens of billions of dollars every year. Despite this investment, HF therapies have limited efficacy in reducing disease progression. Recent insights in fundamental myocyte biology, HF etiologies and pathogenic mechanisms are propelling new strategies to treat and prevent HF. This meeting will explore biologic and technical advances that inform the genetic architecture, molecular pathogenesis and innovative approaches to treat HF. This comes at a time when very large human HF datasets are available and can be interrogated using advanced computational and bioinformatic approaches. Specific aims are to: 1) Consider genes, molecules, signaling pathways and biomarkers involved in systolic and diastolic HF in humans; 2) Explore disease mechanisms underlying HF in model systems; 3) Understand the role of epigenetics, miRNAs and lncRNAs in HF pathogenesis; and 4) Review translational programs in genomic, pharmacologic and cell-based therapeutics to treat HF. The outcomes of this meeting should be far-reaching for basic, translational and clinical communities. The meeting should also provide an excellent training program for junior scientists and serve as a catalyst for collaboration among research, clinical and industrial participants. This meeting is being held in conjunction with the “Cardiac Development, Regeneration and Repair” meeting that provides an outstanding opportunity for joint sessions in genetics, iPSC disease modeling, stem cell therapeutics and epigenetics.
View Scholarships/Awards
Heart failure (HF) is a worldwide epidemic with treatments costing tens of billions of dollars every year. Despite this investment, HF therapies have limited efficacy in reducing disease progression. Recent insights in fundamental myocyte biology, HF etiologies and pathogenic mechanisms are propelling new strategies to treat and prevent HF. This meeting will explore biologic and technical advances that inform the genetic architecture, molecular pathogenesis and innovative approaches to treat HF. This comes at a time when very large human HF datasets are available and can be interrogated using advanced computational and bioinformatic approaches. Specific aims are to: 1) Consider genes, molecules, signaling pathways and biomarkers involved in systolic and diastolic HF in humans; 2) Explore disease mechanisms underlying HF in model systems; 3) Understand the role of epigenetics, miRNAs and lncRNAs in HF pathogenesis; and 4) Review translational programs in genomic, pharmacologic and cell-based therapeutics to treat HF. The outcomes of this meeting should be far-reaching for basic, translational and clinical communities. The meeting should also provide an excellent training program for junior scientists and serve as a catalyst for collaboration among research, clinical and industrial participants. This meeting is being held in conjunction with the “Cardiac Development, Regeneration and Repair” meeting that provides an outstanding opportunity for joint sessions in genetics, iPSC disease modeling, stem cell therapeutics and epigenetics.
View Scholarships/Awards
No registration fees are used to fund entertainment or alcohol at this conference
The meeting will begin on Sunday, April 3 with registration from 16:00 to 20:00 and a welcome mixer from 18:00 to 20:00. Conference events conclude on Thursday, April 7 with a closing plenary session from 17:00 to 19:00, followed by a social hour and entertainment. We recommend return travel on Friday, April 8 in order to fully experience the meeting.
SUNDAY, APRIL 3
MONDAY, APRIL 4
TUESDAY, APRIL 5
WEDNESDAY, APRIL 6
THURSDAY, APRIL 7
FRIDAY, APRIL 8
Conference Program Print | View meeting in 12 hr (am/pm) time
The meeting will begin on Sunday, April 3 with registration from 16:00 to 20:00 and a welcome mixer from 18:00 to 20:00. Conference events conclude on Thursday, April 7 with a closing plenary session from 17:00 to 19:00, followed by a social hour and entertainment. We recommend return travel on Friday, April 8 in order to fully experience the meeting.
SUNDAY, APRIL 3
18:00—20:00
Welcome Mixer
No registration fees are used to fund alcohol served at this function.
08:00—09:00
Welcome and Keynote Address
James A. Spudich,
Stanford University, USA
Mutations to Mechanisms to Therapies
Mutations to Mechanisms to Therapies
08:00—09:00
Welcome and Keynote Address
Peter W. Reddien,
Whitehead Institute, USA
The Role of Muscle Cells in Directing Regeneration in Planarians
The Role of Muscle Cells in Directing Regeneration in Planarians
09:00—11:30
Heart Failure 2016: Syndromes, Mechanisms and Treatments
*
Christine E. Seidman,
Harvard Medical School, USA
*
Hugh Watkins,
University of Oxford, UK
Frank R. Heinzel,
, Germany
What We Don't Know?
What We Don't Know?
Denise Hilfiker-Kleiner,
Medizinische Hochschule Hannover, Germany
Molecular Mechanisms Underlying Peripartum Cardiomyopathy
Molecular Mechanisms Underlying Peripartum Cardiomyopathy
Robert N. Willette,
GlaxoSmithKline, USA
Challenges and Opportunities in Heart Failure Drug Discovery: An Industry Perspective
Challenges and Opportunities in Heart Failure Drug Discovery: An Industry Perspective
Leanne Elizabeth Felkin,
Imperial College London, UK
Short Talk: Recovery of Cardiac Function in Cardiomyopathy due to Titin Truncation
Short Talk: Recovery of Cardiac Function in Cardiomyopathy due to Titin Truncation
Pu Qin,
GlaxoSmithKline, USA
Short Talk: Activation of the Amino Acid Response Pathway Blunts the Effects of Cardiac Stress and Improves Survival
Short Talk: Activation of the Amino Acid Response Pathway Blunts the Effects of Cardiac Stress and Improves Survival
09:00—11:30
Cardiac Lineage Commitment and Specification
*
Christine L. Mummery,
Leiden University Medical Center, Netherlands
Vincent M. Christoffels,
Academic Medical Center, Netherlands
T-Box Transcription Factors in Conduction System Lineage Determination
T-Box Transcription Factors in Conduction System Lineage Determination
Benoit G. Bruneau,
Gladstone Institutes, USA
Transcriptional Regulation of Heart Development and Chromatin Structure
Transcriptional Regulation of Heart Development and Chromatin Structure
Katherine E. Yutzey,
Cincinnati Children's Hospital Medical Center, USA
Epicardial-Derived Lineages in Heart Development and Disease
Epicardial-Derived Lineages in Heart Development and Disease
Alexandre R. Colas,
Sanford-Burnham Medical Research Institute, USA
Short Talk: Id1 Is an Evolutionarily Conserved Master Regulator of Cardiogenic Mesoderm Formation
Short Talk: Id1 Is an Evolutionarily Conserved Master Regulator of Cardiogenic Mesoderm Formation
Daniel M. DeLaughter,
Harvard Medical School, USA
Short Talk: Single Cell Transcriptional Atlas of Cardiac Development
Short Talk: Single Cell Transcriptional Atlas of Cardiac Development
14:30—16:30
Workshop and Panel 1: Molecular Etiology of Heart Failure
*
Norbert Hubner,
Max-Delbrück-Centrum für Molekulare Medizin, Germany
*
Denise Hilfiker-Kleiner,
Medizinische Hochschule Hannover, Germany
Daniel I. Swerdlow,
University College London, UK
Heart FailuRe Molecular Epidemiology for Therapeutic TargetS (HERMES) Consortium: Design of a Collaborative Genetic Meta-Analysis Investigating Causal Pathways in Heart Failure
Heart FailuRe Molecular Epidemiology for Therapeutic TargetS (HERMES) Consortium: Design of a Collaborative Genetic Meta-Analysis Investigating Causal Pathways in Heart Failure
J. Gustav Smith,
Lund University, Sweden
Discovery and Initial Characterization of Genetic Variation on Chromosome 5q22 Associated with Mortality in Heart Failure
Discovery and Initial Characterization of Genetic Variation on Chromosome 5q22 Associated with Mortality in Heart Failure
Lek Wen Tan,
Genome Institute of Singapore, Singapore
Circular RNA Landscape in Human and Mouse Heart
Circular RNA Landscape in Human and Mouse Heart
Anthony Cammarato,
Johns Hopkins University, USA
Influence of Actin Pseudo-Acetylation on in vivo and in vitro Cardiac Performance
Influence of Actin Pseudo-Acetylation on in vivo and in vitro Cardiac Performance
Yasmine Essam Eldin Soliman Aguib,
Aswan Heart Centre, Magdi Yacoub Foundation, Egypt
Defining the Genetic Architecture of Cardiomyopathy within the Egyptian Population
Defining the Genetic Architecture of Cardiomyopathy within the Egyptian Population
Saptarsi M. Haldar,
Amgen Research and Gladstone Institutes / University of California, San Francisco, USA
Therapeutic Targeting of Chromatin-Dependent Signaling in Heart Failure
Therapeutic Targeting of Chromatin-Dependent Signaling in Heart Failure
Selvi Celik,
Lund University, Sweden
Atrial Natriuretic Peptide Expression is Negatively Regulated by a Long Noncoding Antisense RNA Transcript (NPPA-AS1) in Human Cardiomyocytes
Atrial Natriuretic Peptide Expression is Negatively Regulated by a Long Noncoding Antisense RNA Transcript (NPPA-AS1) in Human Cardiomyocytes
Rudolf J. Wiesner,
University of Cologne, Germany
Mosaic Mitochondrial Respiratory Chain Deficiency Causes Cardiac Arrhythmia during Aging
Mosaic Mitochondrial Respiratory Chain Deficiency Causes Cardiac Arrhythmia during Aging
17:00—19:00
Cardiovascular Tissue and Organs on Chips (Joint)
*
Karl Tryggvason,
Karolinska Institutet, Sweden
Thomas Eschenhagen,
University Medical Center Hamburg-Eppendorf, Germany
Dissecting Gene Variant Effects with Tissue Models
Dissecting Gene Variant Effects with Tissue Models
Christopher S. Chen,
Boston University, USA
Mechanoregulation of Form and Function: A Story of Cell Adhesion and the Cytoskeleton
Mechanoregulation of Form and Function: A Story of Cell Adhesion and the Cytoskeleton
Eric N. Olson,
University of Texas Southwestern Medical Center, USA
Myoediting: Correction of DMD by CRISPR/Cas9 Gene Editing
Myoediting: Correction of DMD by CRISPR/Cas9 Gene Editing
Kevin Beussman,
University of Washington, USA
Short Talk: Intracellular Assessment of the Maturation of Excitation-Contraction Coupling in Human Stem Cell-Derived Cardiomyocytes
Short Talk: Intracellular Assessment of the Maturation of Excitation-Contraction Coupling in Human Stem Cell-Derived Cardiomyocytes
19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.
08:00—11:15
Sarcomere Genetics: So What’s New?
*
Norbert Hubner,
Max-Delbrück-Centrum für Molekulare Medizin, Germany
*
Carolyn Ho,
Brigham and Women's Hospital, USA
Stuart Cook,
, Singapore
Disease Mechanisms in Titin Cardiomyopathy
Disease Mechanisms in Titin Cardiomyopathy
Christine E. Seidman,
Harvard Medical School, USA
Genetic (and Other) Antidotes for Cardiomyopathy
Genetic (and Other) Antidotes for Cardiomyopathy
Richard L. Moss,
University of Wisconsin, Madison, USA
Modulation of Sarcomeres via MYBPC3
Modulation of Sarcomeres via MYBPC3
Leslie A. Leinwand,
University of Colorado Boulder, USA
Myosin Myopathies: Pathogenesis and Potential Therapeutics
Myosin Myopathies: Pathogenesis and Potential Therapeutics
Hugh Watkins,
University of Oxford, UK
Sequencing Sarcomeric (and Other Genes) in Cardiomyopathy
Sequencing Sarcomeric (and Other Genes) in Cardiomyopathy
08:00—11:15
Cardiac Morphogenesis and Regeneration
*
Benoit G. Bruneau,
Gladstone Institutes, USA
Jonathan A. Epstein,
University of Pennsylvania, USA
Nuclear Architecture and Cardiac Development
Nuclear Architecture and Cardiac Development
Eldad Tzahor,
Weizmann Institute of Science, Israel
Novel Insights into Cardiac Regeneration
Novel Insights into Cardiac Regeneration
Neil C. Chi,
University of California, San Diego, USA
Cellular and Genetic Dissection of Cardiovascular Development and Regeneration
Cellular and Genetic Dissection of Cardiovascular Development and Regeneration
Ignacio Flores,
Centro Nacional de Investigaciones Cardiovasculares, Spain
Short Talk: Telomerase is Essential for Zebrafish Heart Regeneration
Short Talk: Telomerase is Essential for Zebrafish Heart Regeneration
Tilde Eskildsen,
Odense University Hospital, Denmark
Short Talk: Defining the Earliest Step of Cardiovascular Development Using CRISPR Genome Editing Technology
Short Talk: Defining the Earliest Step of Cardiovascular Development Using CRISPR Genome Editing Technology
17:00—19:00
Systems Dissection of Cardiac Failure
*
Yigal M. Pinto,
University of Amsterdam, Netherlands
Norbert Hubner,
Max-Delbrück-Centrum für Molekulare Medizin, Germany
Dissecting the Genetic Basis of Translational Regulation in Heart Failure
Dissecting the Genetic Basis of Translational Regulation in Heart Failure
Andrew R. Marks,
Columbia University College of Physicians and Surgeons, USA
Towards a Structural Basis of Complex Disorders of the Heart: Calcium Leak and Mitochondria
Towards a Structural Basis of Complex Disorders of the Heart: Calcium Leak and Mitochondria
17:00—19:00
Differentiation and Characterization of Cardiomyocytes from Pluripotent Stem Cells
*
Martina Brueckner,
Yale University School of Medicine, USA
David A. Elliott,
Murdoch Childrens Research Institute, Australia
Generating Cellular Diversity during Human Cardiovascular Development
Generating Cellular Diversity during Human Cardiovascular Development
Bernd K. Fleischmann,
Bonn University, Germany
Assessing Plasticity of Cardiomyocytes Using Pluripotent ES-Cell and Mouse Models
Assessing Plasticity of Cardiomyocytes Using Pluripotent ES-Cell and Mouse Models
Sanjay Sinha,
University of Cambridge, UK
Short Talk: Human Pluripotent Stem Cell-Derived Epicardium Promotes Cardiomyocyte Maturation and Contraction in Tissue-Engineered Cardiac Constructs
Short Talk: Human Pluripotent Stem Cell-Derived Epicardium Promotes Cardiomyocyte Maturation and Contraction in Tissue-Engineered Cardiac Constructs
19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.
08:00—11:15
Aging Hearts
*
Leslie A. Leinwand,
University of Colorado Boulder, USA
*
Gerald W. Dorn, II,
Washington University School of Medicine, USA
Jeffrey Robbins,
Cincinnati Children's Hospital Medical Center, USA
Interplay of Proteotoxicity, Heart Failure and Autophagy
Interplay of Proteotoxicity, Heart Failure and Autophagy
Richard T. Lee,
Harvard University, USA
Systemic Factors and Cardiac Aging
Systemic Factors and Cardiac Aging
Jeffery D. Molkentin,
Cincinnati Children's Hospital Medical Center, USA
Genetic Lineage Tracing Defines Myofibroblast Origin and Function in the Injured Heart
Genetic Lineage Tracing Defines Myofibroblast Origin and Function in the Injured Heart
Joseph A. Hill,
University of Texas Southwestern Medical Center, USA
Autophagy and Heart Disease
Autophagy and Heart Disease
Rolf Bodmer,
Sanford-Burnham Medical Research Institute, USA
Short Talk: Preserving Heart Function despite Obesity, Aging and Parental Diet
Short Talk: Preserving Heart Function despite Obesity, Aging and Parental Diet
Eleonora Adami,
Max Delbrück Center for Molecular Medicine, MDC, Germany
Short Talk: Ribo-Seq Provides a Genome-Wide View of Translational Control in the Diseased Human and Rat Heart
Short Talk: Ribo-Seq Provides a Genome-Wide View of Translational Control in the Diseased Human and Rat Heart
08:00—11:15
Human Pluripotent Stem Cells as Models of Cardiovascular Disease
*
Stacey L. Rentschler,
Washington University School of Medicine, USA
Joseph C. Wu,
Stanford University School of Medicine, USA
Cardiac iPSCs for Disease Modeling and Drug Discovery
Cardiac iPSCs for Disease Modeling and Drug Discovery
Lior Gepstein,
Technion-Israel Institute of Technology, Israel
iPS Modeling of Cardiac Disease: Arrhythmias and Heart Failure
iPS Modeling of Cardiac Disease: Arrhythmias and Heart Failure
Christine L. Mummery,
Leiden University Medical Center, Netherlands
Human iPS Cell Models of Heart and Blood Vessel Disease
Human iPS Cell Models of Heart and Blood Vessel Disease
Kiran Musunuru,
University of Pennsylvania, USA
Genome Editing of Human Pluripotent Stem Cells to Generate Human Cellular Disease Models
Genome Editing of Human Pluripotent Stem Cells to Generate Human Cellular Disease Models
Zhen Ma,
University of California, Berkeley, USA
Short Talk: Biomaterial-Guided Isogenic iPS Disease Modeling of Hypertrophic Cardiomyopathy
Short Talk: Biomaterial-Guided Isogenic iPS Disease Modeling of Hypertrophic Cardiomyopathy
Yuta Yamamoto,
Kyoto University, Japan
Short Talk: Modeling of Long-QT Syndrome Associated with a Calmodulin Mutation using Human Induced Pluripotent Stem Cells
Short Talk: Modeling of Long-QT Syndrome Associated with a Calmodulin Mutation using Human Induced Pluripotent Stem Cells
17:00—19:15
Molecular Aspects of Muscle Cell Contractility and Relaxation
*
Jeffrey Robbins,
Cincinnati Children's Hospital Medical Center, USA
*
Stefan Engelhardt,
Technical University of Munich, Germany
Wolfgang A. Linke,
University of Munster, Germany
Titin Elasticity Impacts Diastolic and Systolic Function
Titin Elasticity Impacts Diastolic and Systolic Function
David Warshaw,
University of Vermont, USA
Molecular Mechanics of MYBPC3
Molecular Mechanics of MYBPC3
Gerald W. Dorn, II,
Washington University School of Medicine, USA
Mitochondrial Triage: Dynamism Determines Quality Control
Mitochondrial Triage: Dynamism Determines Quality Control
Nina de Groot,
University of Amsterdam, Netherlands
Short Talk: Rbm24 Influences the force-Ca2+ Relation in Heart Muscle
Short Talk: Rbm24 Influences the force-Ca2+ Relation in Heart Muscle
Ryan L. Boudreau,
University of Iowa, USA
Short Talk: The Cardiac microRNA-target Interactome Reveals a Novel miR-24:SCN5A:SNP Interaction Associating with Non-Arrhythmic Death in Heart Failure
Short Talk: The Cardiac microRNA-target Interactome Reveals a Novel miR-24:SCN5A:SNP Interaction Associating with Non-Arrhythmic Death in Heart Failure
17:00—19:15
Vascular Development and Disease
Anne C. Eichmann,
Yale University School of Medicine, USA
Signalling in Vascular Development and Function
Signalling in Vascular Development and Function
Stefanie Dimmeler,
University of Frankfurt, Germany
Non-Coding RNAs in Cardiovascular Repair
Non-Coding RNAs in Cardiovascular Repair
Alexandria Afonso,
McMaster University, Canada
Short Talk: Modeling Genetic Determinants of Early-Onset Coronary Artery Disease using Patient-Derived Induced Pluripotent Stem Cells
Short Talk: Modeling Genetic Determinants of Early-Onset Coronary Artery Disease using Patient-Derived Induced Pluripotent Stem Cells
Naoko Koyano-Nakagawa,
University of Minnesota, USA
Short Talk: Feedback Mechanisms Regulate Ets variant 2 (Etv2) Gene Expression and Hematoendothelial Lineages
Short Talk: Feedback Mechanisms Regulate Ets variant 2 (Etv2) Gene Expression and Hematoendothelial Lineages
19:15—20:15
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.
08:00—11:15
Novel Therapeutic Approaches to Fix Broken Hearts (Joint)
*
Joseph C. Wu,
Stanford University School of Medicine, USA
Paul R. Riley,
University of Oxford, UK
Developmental Programming of the Cardiac Lymphatics towards Heart Repair
Developmental Programming of the Cardiac Lymphatics towards Heart Repair
Kenneth D. Poss,
Duke University Medical Center, USA
Endogenous Heart Regeneration Programs
Endogenous Heart Regeneration Programs
Charles E. Murry,
University of Washington, USA
Cardiogenesis with Human Pluripotent Stem Cells
Cardiogenesis with Human Pluripotent Stem Cells
Karl Tryggvason,
Karolinska Institutet, Sweden
Using Laminins to Generate Therapeutic Cardiomyocytes from hES Cells
Using Laminins to Generate Therapeutic Cardiomyocytes from hES Cells
Anna Blice-Baum,
Johns Hopkins University, USA
Short Talk: Titered FOXO Overexpression Maintains Cardiac Proteostasis and Ameliorates Age-Associated Functional Decline
Short Talk: Titered FOXO Overexpression Maintains Cardiac Proteostasis and Ameliorates Age-Associated Functional Decline
Palmer Yu,
Gladstone Institutes, USA
Short Talk: Regeneration of Cardiomyocytes in vivo Using Human Cardiac Reprogramming Factors in a Pig Model
Short Talk: Regeneration of Cardiomyocytes in vivo Using Human Cardiac Reprogramming Factors in a Pig Model
17:00—18:45
Epigenetics and miRNAs: Muscle Failure and Arrhythmia Predisposition
*
Esther E. Creemers,
Academic Medical Center, Netherlands
*
Joseph A. Hill,
University of Texas Southwestern Medical Center, USA
Stefan Engelhardt,
Technical University of Munich, Germany
MiRNAs from Non-Muscle Cells
MiRNAs from Non-Muscle Cells
Roger Foo,
Genome Institute of Singapore, Singapore
What Single Cell Genomics Can Tell Us About Heart Failure
What Single Cell Genomics Can Tell Us About Heart Failure
Yigal M. Pinto,
University of Amsterdam, Netherlands
Role of Cardiac Specific RNA Binding Proteins in Regulating miRNA Function
Role of Cardiac Specific RNA Binding Proteins in Regulating miRNA Function
17:00—18:45
Cardiomyocyte Maturation, Proliferation and Cardiac Remodeling
*
Bernd K. Fleischmann,
Bonn University, Germany
Anthony J. Muslin,
Sanofi Global Research & Development, USA
Signal Transduction Pathways Related to Cardiac Hypertrophy
Signal Transduction Pathways Related to Cardiac Hypertrophy
Jose Luis de la Pompa,
Centro Nacional de Investigaciones Cardiovasculares, Spain
Notch Signaling in Ventricular Chamber Development and Disease
Notch Signaling in Ventricular Chamber Development and Disease
Thomas Braun,
, Germany
The Role of Dedifferentiated Cardiomyocytes in the Diseased Myocardium
The Role of Dedifferentiated Cardiomyocytes in the Diseased Myocardium
19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.
20:00—23:00
Entertainment
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.
*Session Chair †Invited, not yet responded.
We gratefully acknowledge support for this conference from:
Keystone Symposia thanks our Sponsors(s) for generously supporting this meeting:
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We gratefully acknowledge the generous grant for this conference provided by:
We gratefully acknowledge additional support for this conference from:
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American Heart Association's Council on Functional Genomics and Translational Biology |
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Director of Corporate Relations, Email: sarahl@keystonesymposia.org, Phone:+1 970-262-2690 Click here for more information on Industry Support and Recognition Opportunities. If you are interested in becoming an advertising/marketing in-kind partner, please contact: Nick Dua, Senior Director, Communications, Email: nickd@keystonesymposia.org, Phone:+1 970-262-1179 |
