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This meeting took place in 2019
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Imaging Across Scales: Leveraging the Revolution in Resolution (D1)
Organizer(s) Bo Huang and Elizabeth R. Wright
April 7—10, 2019
Snowbird Resort • Snowbird, UT USA
Discounted Abstract Deadline: Dec 11, 2018
Abstract Deadline: Feb 5, 2019
Scholarship Deadline: Dec 11, 2018
Discounted Registration Deadline: Feb 5, 2019
Supported by the Directors' Fund
Summary of Meeting:
Cellular processes are orchestrated by a large number of biomolecules in both spatially and temporally coordinated manners within a small volume. To uncover the underlying organizational principles and their functional relevance, light and electron microscopy are indispensable tools. Light microscopy is exquisitely suited for mapping the spatial localization, temporal dynamics and activity profiles of biomolecules in individual cells to tissues. Advanced electron microscopy technologies support studies for determining the high-resolution structures of isolated biomolecules, as well as biomolecule structure and location within the context of an intact cell. Recent technical breakthroughs have supported the generation of crystal clear pictures and coordination to biological function of biomolecules from the molecular scale to tissue scale. Further technical advancements and biological discoveries call for seamless integration and correlation of different microscopy modalities, labeling methods and analysis/modeling platforms, as well as close communications between technique developers and biologists. Researchers from these very different disciplines, however, do not often sit in the same room and discuss new developments and opportunities for bridging across fields. Instead, their interactions have been limited to brief encounters at sessions of large society meetings and sporadic small symposiums. This meeting aims at bringing together investigators from these research communities in order to stimulate new ideas and forge new collaborations, which will help not only to extend the frontiers of microscopy, but also to bridge new technique developments and their potential applications to addressing the complexity of cell structure and function.
View Scholarships/Awards
Cellular processes are orchestrated by a large number of biomolecules in both spatially and temporally coordinated manners within a small volume. To uncover the underlying organizational principles and their functional relevance, light and electron microscopy are indispensable tools. Light microscopy is exquisitely suited for mapping the spatial localization, temporal dynamics and activity profiles of biomolecules in individual cells to tissues. Advanced electron microscopy technologies support studies for determining the high-resolution structures of isolated biomolecules, as well as biomolecule structure and location within the context of an intact cell. Recent technical breakthroughs have supported the generation of crystal clear pictures and coordination to biological function of biomolecules from the molecular scale to tissue scale. Further technical advancements and biological discoveries call for seamless integration and correlation of different microscopy modalities, labeling methods and analysis/modeling platforms, as well as close communications between technique developers and biologists. Researchers from these very different disciplines, however, do not often sit in the same room and discuss new developments and opportunities for bridging across fields. Instead, their interactions have been limited to brief encounters at sessions of large society meetings and sporadic small symposiums. This meeting aims at bringing together investigators from these research communities in order to stimulate new ideas and forge new collaborations, which will help not only to extend the frontiers of microscopy, but also to bridge new technique developments and their potential applications to addressing the complexity of cell structure and function.
View Scholarships/Awards
No registration fees are used to fund entertainment or alcohol at this conference
The meeting will begin on Sunday, April 7 with registration from 16:00 to 20:00 and a welcome mixer from 18:00 to 20:00. Conference events conclude on Wednesday, April 10 with a closing plenary session from 17:00 to 19:15, followed by a social hour and entertainment. We recommend return travel on Thursday, April 11 in order to fully experience the meeting.
SUNDAY, APRIL 7
MONDAY, APRIL 8
TUESDAY, APRIL 9
WEDNESDAY, APRIL 10
THURSDAY, APRIL 11
Conference Program Print | View meeting in 12 hr (am/pm) time
The meeting will begin on Sunday, April 7 with registration from 16:00 to 20:00 and a welcome mixer from 18:00 to 20:00. Conference events conclude on Wednesday, April 10 with a closing plenary session from 17:00 to 19:15, followed by a social hour and entertainment. We recommend return travel on Thursday, April 11 in order to fully experience the meeting.
SUNDAY, APRIL 7
18:00—20:00
Welcome Mixer
No registration fees are used to fund alcohol served at this function.
08:00—09:00
Welcome and Keynote Address
*
Bo Huang,
University of California, San Francisco, USA
*
Elizabeth R. Wright,
University of Wisconsin-Madison, USA
Xiaowei Zhuang,
Harvard University, USA
Transcriptome and Chromosome Imaging in Single Cells
Transcriptome and Chromosome Imaging in Single Cells
09:00—11:30
Microscopy Techniques I
*
Elizabeth R. Wright,
University of Wisconsin-Madison, USA
Melanie Ohi,
University of Michigan, USA
Using Single Particle cryo-EM to Study Machines Involved in Bacterial Pathogenesis
Using Single Particle cryo-EM to Study Machines Involved in Bacterial Pathogenesis
Coffee Break
Elizabeth Villa,
University of California, San Diego, USA
Opening Windows Into Parkinson’s Disease: Revealing the in situ Structure of a Pathogenic Mutant of LRRK2
Opening Windows Into Parkinson’s Disease: Revealing the in situ Structure of a Pathogenic Mutant of LRRK2
Jennifer Kefauver,
The Scripps Research Institute, USA
Short Talk: Structure of the Human Volume-Regulated Anion Channel
Short Talk: Structure of the Human Volume-Regulated Anion Channel
Anthony Fitzpatrick,
Zuckerman Institute, Columbia University, USA
Short Talk: Ultrafast Electron Tomography
Short Talk: Ultrafast Electron Tomography
17:00—19:00
Microscopy Techniques II
*
Bo Huang,
University of California, San Francisco, USA
Joerg Bewersdorf,
Yale University, USA
3D and Multicolor Live-Cell Super-Resolution Microscopy for Cell Biological Research
3D and Multicolor Live-Cell Super-Resolution Microscopy for Cell Biological Research
Manos Karagiannis,
Massachusetts Institute of Technology, USA
Expansion Microscopy and Other Tools for Imaging at Scale
Expansion Microscopy and Other Tools for Imaging at Scale
Loic A. Royer,
Chan Zuckerberg Biohub, USA
Pushing the Limits of Fluorescence Microscopy with Adaptive Imaging and Deep Learning
Pushing the Limits of Fluorescence Microscopy with Adaptive Imaging and Deep Learning
Hongda Wang,
University of California, Los Angeles, USA
Short Talk: Cross-Modality Super-Resolution in Fluorescence Microscopy Enabled by Deep Learning
Short Talk: Cross-Modality Super-Resolution in Fluorescence Microscopy Enabled by Deep Learning
Jonathan Herrmann,
Stanford University, USA
Short Talk: Time-Resolved Cryo-EM Reveals Multi-Step 2D Protein Crystallization via a Calcium-Mediated Conformational Switch
Short Talk: Time-Resolved Cryo-EM Reveals Multi-Step 2D Protein Crystallization via a Calcium-Mediated Conformational Switch
19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.
08:00—11:15
Molecular Probes I
*
Ru Gunawardane,
Allen Institute for Cell Science, USA
Jin Zhang,
University of California, San Diego, USA
Illuminating the Biochemical Activity Architecture of the Cell
Illuminating the Biochemical Activity Architecture of the Cell
Matthias Wolf,
Okinawa Institute of Science and Technology, Japan
Short Talk: 3D Imaging of Selectively Labeled Drosophila Neurons with Serial Blockface Electron Microscopy and X-Ray Microscopy Using Genetically Encoded Tags
Short Talk: 3D Imaging of Selectively Labeled Drosophila Neurons with Serial Blockface Electron Microscopy and X-Ray Microscopy Using Genetically Encoded Tags
Coffee Break
Clodagh C. O'Shea,
The Salk Institute for Biological Studies, USA
Cracking the Nucleus: Visualizing the Genome at Nucleosome Resolutions and Megabase Scales
Cracking the Nucleus: Visualizing the Genome at Nucleosome Resolutions and Megabase Scales
Adrian R. Ferre-D'Amare,
National Institutes of Health, USA
Short Talk: RNA Analogs of GFP: Aptamers that Induce Fluorescence of Cognate Small-Molecule Ligands
Short Talk: RNA Analogs of GFP: Aptamers that Induce Fluorescence of Cognate Small-Molecule Ligands
Richard Conroy,
NIH Common Fund, USA
Short Talk: The NIH Common Fund Human BioMolecular Atlas Program (HuBMAP)
Short Talk: The NIH Common Fund Human BioMolecular Atlas Program (HuBMAP)
17:00—19:00
Molecular Probes II
*
Clodagh C. O'Shea,
The Salk Institute for Biological Studies, USA
Ru Gunawardane,
Allen Institute for Cell Science, USA
Labeling Endogenous Proteins in iPSCs to Illuminate Stem Cell Organization and Dynamics
Labeling Endogenous Proteins in iPSCs to Illuminate Stem Cell Organization and Dynamics
Xiaokun Shu,
University of California, San Francisco, USA
Visualizing Dynamic Cell Signaling in vivo with a New Class of Fluorescent Reporters
Visualizing Dynamic Cell Signaling in vivo with a New Class of Fluorescent Reporters
Gary G. Borisy,
Forsyth, USA
Mapping Microbiomes at the Micron Scale
Mapping Microbiomes at the Micron Scale
Micah Rapp,
Columbia University in the City of New York, USA
Short Talk: Visualizing the Clustered Protocadherin Self-Recognition Complex
Short Talk: Visualizing the Clustered Protocadherin Self-Recognition Complex
Antonina Roll-Mecak,
NINDS, National Institutes of Health, USA
Short Talk: Severing Enzymes Amplify Microtubule Arrays by Promoting Microtubule Lattice Repair
Short Talk: Severing Enzymes Amplify Microtubule Arrays by Promoting Microtubule Lattice Repair
19:00—20:00
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.
08:00—11:15
Visualizing Cellular and Molecular Structures I
*
Erik M. Jorgensen,
University of Utah, USA
Jan Ellenberg,
European Molecular Biology Laboratory, Germany
Analyzing Nuclear Pores and Chromosomes by Correlative Live, Super-Resolution and Electron Microscopy
Analyzing Nuclear Pores and Chromosomes by Correlative Live, Super-Resolution and Electron Microscopy
Ralf Jungmann,
Max Planck Institute of Biochemistry, Germany
Super-Resolution Microscopy with DNA Molecules: Towards Localizomics
Super-Resolution Microscopy with DNA Molecules: Towards Localizomics
Colin Comerci,
Stanford University, USA
Short Talk: STED Microscopy Reveals Protein Self-Assembly Drives Localized Surface Layer Growth in C. crescentus
Short Talk: STED Microscopy Reveals Protein Self-Assembly Drives Localized Surface Layer Growth in C. crescentus
Coffee Break
Elizabeth R. Wright,
University of Wisconsin-Madison, USA
Correlative Cryo-EM – Light Microscopy of Virus Entry/Fusion
Correlative Cryo-EM – Light Microscopy of Virus Entry/Fusion
Bram J. Koster,
Leiden University Medical Center, Netherlands
Zooming in on Cells and Molecules with Correlative Light and Electron Microscopy
Zooming in on Cells and Molecules with Correlative Light and Electron Microscopy
Elizabeth A. Campbell,
Rockefeller University, USA
Short Talk: Cryo-EM Structures of an RNA Polymerase Promoter Melting Intermediate Elucidate DNA Unwinding
Short Talk: Cryo-EM Structures of an RNA Polymerase Promoter Melting Intermediate Elucidate DNA Unwinding
Shrawan Kumar Mageswaran,
California Institute of Technology, USA
Short Talk: Electron Cryotomography of Mitochondrial Constriction Sites Urges Reevaluation of Mechanisms for Fission
Short Talk: Electron Cryotomography of Mitochondrial Constriction Sites Urges Reevaluation of Mechanisms for Fission
17:00—19:00
Visualizing Cellular and Molecular Structures II
*
Jan Ellenberg,
European Molecular Biology Laboratory, Germany
Martin Pilhofer,
ETH Zurich, Switzerland
Multiscale Models of Bacterial Cell-Cell Interactions
Multiscale Models of Bacterial Cell-Cell Interactions
Erik M. Jorgensen,
University of Utah, USA
Live Multicolor Super-Resolution Microscopy
Live Multicolor Super-Resolution Microscopy
Yuki Hamamura,
University of Hamburg, Germany
Short Talk: Analyzing the Dynamics of Meiotic Chromosomes Gives Mechanistic Insights into the Regulation of Meiotic Progression
Short Talk: Analyzing the Dynamics of Meiotic Chromosomes Gives Mechanistic Insights into the Regulation of Meiotic Progression
Collin Kieffer,
University of Illinois at Urbana-Champaign, USA
Short Talk: Moving Pathology into the 21st Century: 3D Multiscale Imaging of HIV in Tissues
Short Talk: Moving Pathology into the 21st Century: 3D Multiscale Imaging of HIV in Tissues
19:15—20:15
Social Hour with Lite Bites
No registration fees are used to fund alcohol served at this function.
20:00—23:00
Entertainment
Entertainment is not subsidized by conference registration fees nor any U.S. federal government grants. Funding for this expense is provided by other revenue sources.
*Session Chair †Invited, not yet responded.
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