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This meeting took place in 2002

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Protein Phosphorylation and Mechanisms of Cellular Regulation (X2)

Organizer(s) Natalie G. Ahn and Gary L. Johnson
March 5—10, 2002
Taos Convention Center (meeting only) • Taos, New Mexico USA
Abstract Deadline: Nov 5, 2001
Late Abstract Deadline:
Scholarship Deadline:
Early Registration Deadline: Jan 4, 2002

Sponsored by Amgen Inc.

Summary of Meeting:
This meeting will focus on the control of signal transduction and cell cycle control by phosphorylation and posttranslational modifications, featuring pathways which are well studied and new mechanisms which are emerging. One aim is to report new concepts and discoveries on mechanisms for cellular regulation by signaling molecules, as well as creative approaches towards the design and development of highly specific inhibitors for these targets. Our goal is to provide investigators and students with a broad yet comprehesive understanding of new discoveries in this field using biochemical, structural and genetic strategies. A second aim is to promote novel experimental approaches for participants to use in their research. Plenary talks will highlight innovative strategies towards problems in signal transduction, including monitoring global cellular responses by genomics, proteomics and database mining, new strategies for protein engineering, and fluorescence based assays for monitoring signal transduction in single cells. A workshop will also be held on practical aspects of applying genomics, proteomics and bioinformatics to research problems. The meeting will be held jointly with a Keystone Symposium on "Wnt and Beta-catenin Signaling in Development and Disease", which discusses mechanisms by which the Wnt signaling pathway is regulated, ranging from basic research to clinical applications. Participants can choose between concurrent talks in each program, and two evening sessions will be held jointly, providiing a venue to learn perspectives at the forefront of each discipline, and to establish new contacts and collaborations.

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No registration fees are used to fund entertainment or alcohol at this conference

Conference Program    Print  |   View meeting in 12 hr (am/pm) time



Rio Grande D

Rio Grande A-C

Keynote Address (Joint). Address Sponsored in part by Genentech, Inc.

Roeland Nusse, HHMI/Stanford University, USA
Signaling by Wnt Proteins

Roger Y. Tsien, HHMI/University of California, San Diego, USA
Signal Transduction Visualized with Genetically Encoded Indicators



Cell Proliferation. Session Sponsored in part by Schering-Plough Research Institute

Rio Grande C-D
* Melanie H. Cobb, University of Texas Southwestern Medical Center, USA
ERK's in Progress

Beverly J. Errede, University of North Carolina at Chapel Hill, USA
Regulation of MAPK Activation Module Assembly

Marc K. Saba-El-Leil, Institut de Recherches Cliniques de Montreal, Canada
Short Talk: Targeted Inactivation of the Erk2 MAP Kinase Gene in the Mouse

Carol A. Lange, University of Minnesota, USA
Short Talk: MAPK Couples Multiple Functions of Human Progesterone Receptors: Degradation, Transcription, and Nuclear Localization

Kun-Liang Guan, University of California, San Diego, USA
Regulation of the Raf Kinase

Michael A. White, University of Texas Southwestern Medical Center, USA
Short Talk: Critical Contribution of Linker Proteins to Raf Kinase Activation

Meera V. Sundaram, University of Pennsylvania, USA
Short Talk: C. elegans ksr-1 and ksr-2 have both Unique and Redundant Functions and are Required for MPK-1 Phosphorylation

Larry Feig, Tufts University School of Medicine, USA
Short Talk: A Novel Kinase-Independent Function of PDK1: Allosteric Activator of an Exchange Factor for the Ral-GTPase

Wnt/Beta-Catenin Pathway in Model Organisms

Rio Grande A-B
* Roeland Nusse, HHMI/Stanford University, USA

Lilianna Solnica-Krezel, Vanderbilt University, USA
Short Talk: Regulation of Cell Polarity During Zebrafish Gastrulation

Yasuhiko Kawakami, The Salk Institute for Biological Studies, USA
Short Talk: Wnt Signaling Controls Limb-Type Specification in Vertebrate Embryos

Karl H. Willert, University of California, San Diego, USA
Short Talk: Purification and Biochemical Characterization of a Wnt Protein

Jianbo Wang, University of California, San Diego, USA
Short Talk: The Murine Dishevelled 2 Gene is Essential for Neural Tube Closure

Bruce A. Bowerman, University of Oregon, USA
Using Conditional Mutations and RNA Interference to Identify Genes that Interact with a Non-Canonical Wnt Pathway in the Nematode Caenorhabditis elegans

Mariann Bienz, MRC Laboratory of Molecular Biology, UK
Short Talk: A New Core Component of the Wnt Signaling Pathway

Adrian John Harwood, University College London, UK
Short Talk: A Non-Metazoan GSK-3/beta-Catenin Signaling Pathway

Janet Heasman, Cincinnati Children's Research Foundation, USA
Maternal XTcf3 Prevents the Ectopic Activation of Dorsal Zygotic Genes in the Early Xenopus Embryo

James I. Kim, Massachusetts General Hospital, USA
Short Talk: Array Analysis of Gene Expression Profiles During Neural Induction and Organizer Formation in Xenopus.

Coffee Break

Poster Setup

Poster Viewing

Workshop: Post-Genomic Methods

Rio Grande C-D
* Natalie G. Ahn, University of Colorado, USA

Mark W. Geraci, University of Colorado, USA
Expression Analysis to Study Disease Pathogenesi

Katheryn A. Resing, University of Colorado, USA
Mapping Covalent Modifications on Proteins

Huilin Zhou, Institute for Systems Biology, USA
Quantitative Proteomic: New Technologies and Their Applications

Michael B. Yaffe, Massachusetts Institute of Technology, USA
Scansite: Motif-Based Profile Scanning

Coffee & Snacks Available

Adhesion, Cell Shape and Migration

Rio Grande C-D
* David A. Cheresh, University of California, San Diego, USA
Signaling by Angiogenic Growth Factors

Morag Park, McGill University, Canada
Combinatorial Signals Involved in Epithelial Cell Dispersal and Invasion

Michael P. Sheetz, Mechanobiology Institute, National University of Singapore, Singapore
Force Sensing by the Cell Cytoskeleton: Roles of Tyrosine Phosphatases/Kinases

Kozo Kaibuchi, Nagoya University Graduate School of Medicine, Japan
Regulation of Neuronal Morphology by Rho/Rho-Kinase

Audrey G. Minden, Rutgers University, USA
Short Talk: Regulation of Cell Morphology by PAK5, a New Brain Specific Kinase

Beta-Catenin Signaling and Cancer

Rio Grande A-B
* Paul G. Polakis, Genentech, Inc., USA
The Involvement of Wnt Signaling in Human Cancer

Wendy J. Fantl, Chiron Corporation, USA
Short Talk: Beta-Catenin Regulates Vascular Endothelial Growth Factor (VEGF-A) Expression in Colon Cancer

Hans C. Clevers, Hubrecht Institute, Netherlands
TCF Factors, Mediators of Wnt Signaling

Alicia Collado-Hidalgo, University of California, Los Angeles, USA
Short Talk: Wnt and TGF-beta Cooperation in Mammary Tumor Progression

David A. Jones, University of Utah, USA
Microarray Analysis of Colorectal Cancer and Signal Transduction Pathways

Joyce A. Schroeder, Mayo Clinic-Scottsdale, USA
Short Talk: ErbB/b-Catenin Complex Formation is Specific to Both Transgenic Mammary Gland Carcinoma and Human Breast Cancer Metastases.

Welcome Mixer

Poster Session 1




Second Messengers. Session Sponsored in part by BD Biosciences Pharmingen

Rio Grande C-D
* Susan S. Taylor, University of California, San Diego, USA
Dynamics of PKA

Franz Hofmann, Technische Universität München, Germany
Signaling Through NO and cGMP-Dependent Protein Kinase

Dario Alessi, University of Dundee, UK
Signal Transduction Downstream of PI3 Kinase

Tian Xu, Yale University School of Medicine, USA
Cell Growth, Proliferation, and the Control of Organ Size in Drosophila

John D. Scott, University of Washington, USA
Molecular Organization of Cytoskeletal Transduction Complexes

Mark L. Dell'Acqua, University of Colorado, USA
Short Talk: Imaging a Ternary Kinase-AKAP-Phosphatase Signaling Complex in Living Cells Using Fret Microscopy

Wei-Jen Tang, University of Chicago, USA
Short Talk: Activation of the Anthrax Adenylyl Cyclase Exotoxin by Calmodulin

Beta-Catenin-Independent Wnt and Frizzled Signaling

Rio Grande A-B
* Jeffrey D. Axelrod, Stanford University, USA
Asymmetry and Signal Amplification in Frizzled Mediated Planar Cell Polarity Signaling

Maiyon Park, University of Washington, USA
Short Talk: The Planar Cell Polarity Gene Strabismus Encodes a Dishevelled-Associated Protein that Regulates Cell Behavior and Cell Fate in Vertebrate Embryos

Malini Sen, University of California, San Diego, USA
Short Talk: Wnt Signaling in Rheumatoid Arthritis

Cliff Tabin, Harvard Medical School, USA
Wnt-14 Plays a Pivotal Role in Inducing Synovial Joint Formation in the Developing Appendicular Skeleton

Shoukat Dedhar, British Columbia Cancer Research Centre, Canada
Short Talk: Molecular Basis for the Regulation of Nuclear Beta-Catenin by the Tumor Suppressor PTEN

Alan R. Kimmel, National Institutes of Health, USA
Short Talk: The Frizzled-Domain cAMP Receptors Antagonistically Regulate Differential Tyrosine Phosphorylation and Activity of GSK3 to Orchestrate Cell Fate Specification and Cell Migration.

Coffee Break

Poster Setup

Rio Grande
Poster Viewing

Rio Grande
Coffee & Snacks Available

Rio Grande
Integration of Signaling Pathways (Joint)

* Joan Massagué, Memorial Sloan Kettering Cancer Center, USA
Driving and Integrating TGFbeta Signaling

Jim Woodgett, Mount Sinai Hospital, Canada
Regulation of Glycogen Synthase Kinase-3 and Protein Kinase B

Bart De Strooper, University College London, UK
Where Notch and Wnt Signaling Meet: The Presenilin Hub

Michael P. Snyder, Stanford University School of Medicine, USA
Global Analysis of Yeast Proteins Using Protein Chips

Welcome Mixer

Rio Grande
Poster Session 2

Rio Grande



Cell Cycle and Metabolism. Session Sponsored by Wyeth/Genetics Institute

Rio Grande C-D
* Jeremy Thorner, University of California, Berkeley, USA
Hsl7, a Negative Regulator of the Swe1 Protein Kinase, Shuttles Between the Spindle Pole Body and the Septin Ring in the Budding Yeast Saccharomyces cerevisiae.

Eisuke Nishida, Kyoto University, Japan
Spatiotemporal Control of M Phase Regulators

Brenda Joyce Huneycutt, University of Colorado at Boulder, USA
Short Talk: Understanding Mps1p Function Using Chemical Genetics

Kenji Fukasawa, H Lee Moffitt Cancer Center and Research Institute, USA
Short Talk: Phosphorylation of Nucleophosmin (NPM/B23) by CDK2/Cyclin E and Its Role in Centrosome Duplication

J. Wade Harper, Harvard Medical School, USA
Phosphorylation-Dependent Ubiquitization via the SCF Ubiquitin Ligase Pathway

Bei B. Zhang, Pfizer Inc., USA
Discovery of Small Molecule Insulin Mimetic Agents with Anti-Obesity and Insulin Sensitizing Effects

Rina Plattner, Duke University Medical Center, USA
Short Talk: Novel Link Between the c-Abl Nonreceptor Tyrosine Kinase and Phosphoinositide Signaling via PLC-gamma1

Jie Chen, University of Illinois, Urbana-Champaign, USA
Short Talk: Regulation of mTOR Signaling by Phosphatidic Acid and Phospholipase D Pathways

Beta-Catenin Signaling and Target Genes

Rio Grande A-B
Jeffery R. Barrow, Brigham Young University, USA
The Role of Wnt/beta-Catenin Signaling in the Establishment and Maintenance of the Apical Ectodermal Ridge

Joerg Huelsken, Ecole Polytechnique Fédérale de Lausanne, Switzerland
Short Talk: Beta-Catenin Controls Stem Cell Differentiation in the Skin

Marian Waterman, University of California, Irvine, USA
Expression of LEF/TCFs in Colon Cancer

Michael Kahn, Pacific Northwest Research Foundation, USA
Short Talk: Small Molecule Inhibitors of Tcf-4/Catenin Transcription

William I. Weis, Stanford University, USA
Biochemical and Structural Analysis of beta-Catenin and its Interactions

Wenqing Xu, University of Washington, USA
Short Talk: Structural Basis of beta-Catenin/Tcf and beta-Catenin/ICAT Interactions

* Jacqueline S. Papkoff, JSP3 Consulting, USA
A Genomics Approach for Identification and Validation of Therapeutic Targets in the Beta-Catenin Signal Transduction Pathway

Marta Ines Gallego, National Institutes of Health, USA
Short Talk: Targeted Expression of HGF in Mouse Mammary Epithelium Induces Squamous Metaplasias and Adenocarcinomas Through the Stabilization of Beta-Catenin.

Steve W. Wilson, University College London, UK
Wnt Signals Regulate Both Mesodermal Cell Movements and Anterior-Posterior Patterning of the Brain in the Zebrafish Embryo

Thomas Schwarz-Romond, European Molecular Biology Organization (EMBO), Germany
Short Talk: The Novel Ankyrin Repeat Protein Diversin Acts in the Wnt Signalling Pathway and Controls Embryonic Axis Formation

Coffee Break

Poster Setup

Rio Grande
Poster Viewing

Rio Grande
Coffee & Snacks Available

Rio Grande
Development (Joint)

* Elaine V. Fuchs, Rockefeller University, USA
Wnt Signaling in the Skin

Eric N. Olson, University of Texas Southwestern Medical Center, USA
Signaling Pathways that Control Muscle Development

Rolf Kemler, Max Planck Institute, Germany
Beta-catenin Signaling in Early Mouse Development

Marc Vidal, Dana-Farber Cancer Institute, USA
Toward a Proteome Atlas for C. elegans

Ilaria Rebay, Whitehead Institute for Biomedical Research, USA
Short Talk: Eyes Absent Mediates Cross-Talk Between a Network of Retinal Determination Genes and the Receptor Tyrosine Kinase Signaling Pathway

Welcome Mixer

Rio Grande
Poster Session 3

Rio Grande



Stress and Survival. Session Sponsored by Wyeth/Genetics Institute

Rio Grande C-D
* Gary L. Johnson, UNC Chapel Hill, USA
Cellular Functions of MEKK

Michael Karin, University of California, San Diego, USA
IKK – A Master Regulator of Innate and Adaptive Immune Responses

Jiahuai Han, The Scripps Research Institute, USA
MAP Kinase Kinase-Independent Activation of p38alpha Mediated by TAB1-Dependent Autophosphorylation of p38alpha

Elizabeth J. Goldsmith, University of Texas Southwestern Medical Center Dallas, USA
MAP Kinase Interactions

Roger J. Davis, HHMI/University of Massachusetts Medical School, USA
Signal Transduction by JNK

Hideki Nishitoh, University of Tokyo, Japan
Short Talk: The Role of ASK1 in Stress Signaling

Short Talk: Small Molecule Inhibitors of JNK Inhibit Tumor Growth

Regulation of Wnt Receptors

Rio Grande A-B
* Scott B. Selleck, University of Arizona, USA
Positive and Negative Regulation of Multiple Growth Factor Signaling Pathways by Cell Surface Proteoglycans

Julie T. Tai, CV Therapeutics, USA
Short Talk: Down Regulation of Frzb1 and the Activation of Wnt/beta-Catenin Signal Transduction Pathway in Rat Balloon Vascular Injury Model

Craig C. Malbon, Stony Brook University, USA
Frizzled Signaling and Heterotrimeric G-Proteins

Jeanine M. D'Armiento, Columbia University, USA
Short Talk: sFRP1 Expression Induces MMP and is Associated with Pulmonary Emphysema

Stuart A. Aaronson, Mount Sinai Medical Center, USA
Dkk-1 and sFRP Wnt Inhibitors

Daniel Pinto, Netherland Institute for Develepmental Biology, Netherlands
Short Talk: Consequences of Targeted Expression of Dkk-1, a Specific Inhibitor of the Wnt/Beta-Catenin Signaling Pathway, in the Intestinal Mucosa of Transgenic Mice

Xi He, Harvard Medical School, USA
Wnt Signaling Pathways in Early Vertebrate Embryogenesis

J. Lawrence Marsh, University of California, Irvine, USA
Short Talk: Cross Regulation of Wnts and BMPs and Implications for Oncogenesis

Junichi Hanai, Beth Israel Deaconess Medical Center, USA
Short Talk: Endostatin Functions by Inhibiting beta-Catenin Signaling.

Coffee Break

Coffee & Snacks Available

Issues Arising: Emerging Concepts and Technologies

Rio Grande C-D
* Nancy L. Allbritton, University of Washington, USA
Measurement of Enzyme Activation in Single Cells

Jonathan D. Violin, Trevena Inc., USA
Short Talk: Spatiotemporal Imaging of Conformational Changes of Protein Kinase C

Kevan M. Shokat, University of California, San Francisco, USA
Unnatural Ligands for Engineered Receptors: New Tools for Chemical Genetics

Andrew J. Link, Vanderbilt University School of Medicine, USA
Comprehensive Analysis of Protein Complexes Using Mass Spectrometry

Forest M. White, Massachusetts Institute of Technology, USA
Short Talk: Phosphoproteome Analysis by Mass Spectrometry: Application to S. cerevisiae

Peter J. Kennelly, Virginia Polytechnic Institute & State University, USA
Short Talk: A Protein Kinase from the Archaea (Archaebacteria)

Scott D. Seiwert, Alios BioPharma, Inc., USA
Short Talk: Monitoring Protein Phosphorylation with Array-Compatible Reagents

Interactions of Wnt Pathway Components

Rio Grande A-B
* Mark Peifer, University of North Carolina at Chapel Hill, USA
Cell Adhesion and Cytoskeletal Regulation in Embryogenesis and Oncogenesis

Ken-Ichi Takemaru, Stony Brook University, USA
Short Talk: Chibby, a Novel beta-Catenin-Associated Antagonist of the Wnt/Wingless Pathway

Akira Kikuchi, Hiroshima University, Japan
Regulation of beta-Catenin Signaling by Idax and Axam

Yoshimi Endo, NCI, National Institutes of Health, USA
Short Talk: Dickkopf-1 and Dickkopf-2 Inhibit the Wnt-Canonical Pathway in Metanephric Mesenchyme

Eric R. Fearon, University of Michigan Medical Center, USA
Role of APC and Catenin Defects in Cancer

Juergen Behrens, Nikolaus-Fiebiger Center, Germany
Short Talk: Negative Feedback Loop of Wnt Signaling Through Upregulation of Conductin/Axin2 in Colorectal and Liver Tumors

Welcome Mixer





*Session Chair †Invited, not yet responded.

We gratefully acknowledge the generous grant for this conference provided by:

National Institutes of Health

Grant No. 1 R13 CA94350-01

We gratefully acknowledge additional support for this conference from:

BD Technologies
Educational donation provided by Amgen

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