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This meeting took place in 2007

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Imaging Immune Responses (J7)

Organizer(s) Ronald N. Germain and Ellen A. Robey
February 25—March 1, 2007
Keystone Resort • Keystone, CO USA
Abstract Deadline: Oct 25, 2006
Late Abstract Deadline: Nov 27, 2006
Scholarship Deadline: Oct 25, 2006
Early Registration Deadline: Dec 26, 2006

Supported by the Director's Fund

Summary of Meeting:
The cellular events leading to host defense and immunopathology are dynamic in nature and involve the migration, transient arrest, and redistribution of various hematopoietic cells types between blood, secondary lymphoid organs, and peripheral tissues. For this reason, a great deal of excitement has been generated by the recent application of miniaturized versions of clinical imaging tools such as PET scanning and MRI, confocal and especially multiphoton microscopy, and luminescence imaging to living animals, permitting direct spatiotemporal analysis of immune cells within complex tissue environments. However, standards for data analysis and interpretation are just emerging, as are improved methods that bridge the resolution and time gaps between the various methods, allowing analyses to begin with initiation of a response and to follow it through to pathogen/tumor clearance or induction of autoimmune pathology. This meeting will bring together experts who will describe ongoing and future applications of these new imaging methods and provide the attendees with an understanding of the new biological paradigms that are emerging from such investigations. Speakers will discuss both basic and clinically-related advances, including cell-cell interactions in the development of humoral and cell-mediated immune responses, the events underlying autoimmune pathology in models of rheumatoid arthritis and diabetes, the trafficking of hematopoietic stem cells following grafting, signaling events in the thymus, bone marrow, and secondary lymphoid tissues, the sites of Treg activity, and the interplay between tumor cell growth and immune cell effector activity following anti-cancer vaccination.

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Conference Program    Print  |   View meeting in 12 hr (am/pm) time



Shavano Peak Foyer

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Keynote Address

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Scott E. Fraser, University of Southern California, USA
Observing Dynamic Cell Behavior in Complex Tissues

Keynote Address

Jacques F. Banchereau, The Jackson Laboratory for Genomic Medicine, USA
Taking Dendritic Cells into Medicine



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Antigen Presentation and the Initiation of T Cell Responses

Grays Peak 2-3
* Ronald N. Germain, NIAID, National Institutes of Health, USA
Directed Cell-Cell Interactions in the Development of Adaptive Immunity

Jean M. Davoust, Hôpital Saint-Vincent-de-Paul, France
Langerhans Cell Trafficking and Antigen Capture in the Skin

Ulrich H. Von Andrian, Harvard Medical School, USA
Cell Entry and Interaction within Lymphoid Tissues

Marc K. Jenkins, University of Minnesota Medical School, USA
Imaging Peptide:MHC II Production During the Primary Immune Response

Pathogen Recognition Pathways

* Takashi Fujita, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan
Triggering Antiviral Responses and Cell Regulation by the Cytoplasmic RNA Helicase RIG-I

Akira Shizuo, Osaka University, Japan
TLR-Dependent and Independent Anti-Viral Responses

Michel Gilliet, University Hospital of Lausanne, Switzerland
Short Talk: Activation of Plasmacytoid Dendritic Cell Precursors by an Endogenous Peptide

Dana Philpott, University of Toronto, Canada
NOD Pathway for Pathogen Sensing

Teunis B.H. Geijtenbeek, University of Amsterdam, Netherlands
Short Talk: DC-SIGN Modulates TLR-Induced Immune Responses in Dendritic Cells through Acetylation of NF-kappaB-Subunit p65

Caetano Reis e Sousa, Francis Crick Institute, UK
Activation of DC by Yeast

Coffee Break

Shavano Peak Foyer
Poster Setup

Crestone/Red Cloud
Poster Viewing

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Workshop 1: Intravital Multiphoton Imaging

Grays Peak 2-3
* Mark J. Miller, Washington University School of Medicine, USA
Multi-photon Imaging of Antigen Presentation During Listeria monocytogenes Infection

* Philippe Bousso, Institute Pasteur, France
Cellular Orchestration of T Cell Priming In Vivo

Ann M. Haberman, Yale University School of Medicine, USA
Visualization of Germinal Center B Cell Dynamics In Vivo

Sarah E. Henrickson, Children's Hospital of Philadelphia, USA
T Cell Sensing of Antigen Avidity Determines the Kinetics and Character of T Cell-Dendritic Cell Interactions in Lymph Nodes

Reinhold Förster, Hannover Medical School, Germany
CCR7-Ligands Stimulate the Intranodal Motility of T Lymphocytes in vivo

Joost B. Beltman, Leiden University, Netherlands
Lymph Node Topology Dictates T Cell Migration Behavior

Thorsten R. Mempel, Massachusetts General Hospital, USA
Conduits Mediate Antigen Transport to Follicular Dendritic Cells in Lymph Nodes

Michio Tomura, Kyoto University Graduate School of Medicine, Japan
Following Cells In Vivo Using Photoconverting Protein KAEDE

Workshop 1: Signaling in Pathogen Recognition

* Tsuneyasu Kaisho, RIKEN Research Center for Allergy and Immunology, Japan
Critical Involvement of IkappaB kinase-alpha in TLR7/9-Induced Type I IFN Production

Emmanuel Derudder, CBR Institute for Biomedical Research, USA
Roles for Canonical and Alternative NF-kappaB Activation Pathways in Dendritic Cells

Esther C. de Jong, Academic Medical Center, Netherlands
Non-Canonical NF-kappaB Signaling in Dendritic Cells is Required for Indoleamine 2,3-Dioxygenase (IDO) Induction and Immune Regulation

Ranjeny Thomas, University of Queensland, Australia
RelB-Competent Dendritic Cells Suppress Autoimmune Inflammatory Disease

Hiromitsu Hara, Saga University, Japan
CARD9 is Essential for Myeloid Cell Activation through ITAM-bearing Receptors

Yen-Michael S. Hsu, University of Texas MD Anderson Cancer Center, USA
CARD9 is Required for Innate Immune Responses Against Intracellular Pathogens

Coffee Available

Shavano Peak Foyer
Intracellular Signaling by Lymphoid Cells Analyzed in situ

Grays Peak 2-3
Mark M. Davis, Stanford University School of Medicine, USA
The Role of Endogenous Peptides in T Cell Activation and Thymic Selection

Richard S. Lewis, Stanford University School of Medicine, USA
A Molecular Mechanism for Sustained Calcium Signaling During T Cell Activation

* Doreen A. Cantrell, University of Dundee, UK
Spatial/Temporal Analysis of Inositol Lipid Metabolism During T Lymphocyte Activation

Signaling in Pathogen Recognition

Marco Colonna, Washington University School of Medicine, USA
Cross Talks between TLRs and ITAM-Dependent Receptors in DC

Jürg Tschopp, University of Lausanne, Switzerland
NLRs and the Inflammasome: Danger Sensing Complexes Involved in Inflammatory Diseases

You-Me Kim, KAIST, South Korea
Short Talk: the Interaction Between the ER Membrane Protein UNC93B and Toll-like Receptors 3, 7, and 9 is Crucial for TLR Signaling

* Anne O'Garra, Francis Crick Institute, UK
Molecular Mechanisms for the Regulation of IL-10 Expression in Dendritic Cells, Macrophages and T Cells

Social Hour with Lite Bites

Crestone/Red Cloud
Poster Session 1

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B Cell Migration and the Induction of Humoral Immune Responses

Grays Peak 2-3
* Jason G. Cyster, HHMI/University of California, San Francisco, USA
Cell Migration Dynamics During the B Cell Immune Response

Philippa C. Marrack, HHMI/National Jewish Health, USA
The Effects of Adjuvants on B and T Cells

John H. Kehrl, NIAID, National Institutes of Health, USA
Regulation of B Cell Motility in Lymph Nodes

Michel C. Nussenzweig, HHMI/Rockefeller University, USA
Imaging Germinal Centers

Cross-Talk with Other Cells

* Kenneth L. Rock, University of Massachusetts Medical School, USA
How Dying Cells Alert the Immune System to Danger

Laurence Zitvogel, Institut Gustave Roussy, France
DC and NK Cells

Vincenzo Cerundolo, University of Oxford, UK
DC and NKT cells

Anna M. Keller, Netherlands Cancer Institute, Netherlands
Short Talk: Costimulatory Ligand CD70 is Delivered to the Immunological Synapse by Shared Intracellular Trafficking with MHC Class II

Yatin M. Vyas, Children's Hospital of Pittsburgh, USA
Short Talk: Antiparallel Segregation of Notch Components in the Immunological Synapse Directs Reciprocal Signaling in Allogeneic Th:DC Conjugates

Yvette van Kooyk, Vrije University Medical Center, Netherlands
Regulation of DC Cross Talk with other Immune Cells by C-Type Lectins and Carbohydrates

Coffee Break

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Poster Setup

Crestone/Red Cloud
Poster Viewing

Crestone/Red Cloud
Workshop 2: New Technologies for Functional, High Resolution Imaging

Grays Peak 2-3
* Bridget S. Wilson, University of New Mexico Health Science Center, USA
High Resolution Imaging Methods to Probe Fc[epsilon]RI Membrane Organization and Behavior

Pavel Tolar, Francis Crick Institute, UK
Imaging the B-Cell Receptor Signaling in the Immunological Synapse

Carles Rentero, University of Barcelona, Spain
Membrane Organization at T Cell Activation Sites and Immunological Synapse

Valarie A. Barr, NCI, National Institutes of Health, USA
Cbl-Dependent Internalization of TCR-Induced, LAT-Nucleated Signaling Complexes

Aaron K. Neumann, University of New Mexico, USA
Distribution and Lateral Mobility of DC-SIGN on Immature Dendritic Cells

David M. Haaland, Sandia National Laboratories, USA
Hyperspectral Confocal Imaging of Live Cell Immune Response to Cytokines

Matthias Gunzer, Helmholtz Centre for Infection Research, Germany
The Power of Single- and Multibeam 2-Photon Microscopy for High-Resolution and High-Speed Deep Tissue and Intravital Imaging

Workshop 2: Intercellular Interactions Regulating DC Function

* Shannon J. Turley, Genentech, Inc., USA
Presentation of Intestinal Self by Dendritic Cells and Lymph Node Stroma

Ena Ladi, University of California, Berkeley, USA
Visualizing Dendritic Cell Interactions of Migrating Thymocytes in Real-time Using Two-Photon Microscopy

John P. Driver, The Jackson Laboratory, USA
Activated NKT Cells Inhibit Autoimmune Diabetes Through Tolerogenic Maturation of Dendritic Cells

Flavio A. Salazar Onfray, Institute of Biomedical Sciences, Chile
Functional Gap Junctions Facilitate Melanoma Antigen Cross-Presentation in Human Dendritic Cells and are Recruited to the Immunological Synapses Contributing to T Cell Activation

Lesley Ann Smyth, Kings College London, UK
A Novel Pathway of Antigen Presentation by Dendritic Cells; Implications for Infectious Diseases

Payal Watchmaker, University of Pittsburgh, USA
Memory CD8+ T Cells Protect Dendritic Cells from CTL-Mediated Killing: Helper Function of Memory CD8+ T Cells

Fabienne Brilot, Rockefeller University, USA
NK Cell Survival Mediated Through the Regulatory Synapse With Dendritic Cells Requires IL-15Ralpha

Coffee Available

Shavano Peak Foyer
Lymphocyte Behavior Outside the Lymph Node

Grays Peak 2-3
* Ellen A. Robey, University of California, Berkeley, USA
Cell Interactions and Migration in the Thymus

Matthew F. Krummel, University of California, San Francisco, USA
Visualizing Cellular Decision-Making in Lymphocytes

Ute Frevert, New York University School of Medicine, USA
Intravital Imaging of the Liver Phase of a Malaria Infection

Mark C. Coles, University of Oxford, UK
Imaging Lymphoid Tissue Development and Maturation

DC Migration and Antigen Uptake

* Colin Watts, University of Dundee, UK
Regulation of Cytoskeleton during DC Migration and Antigen Uptake

Ruslan Medzhitov, HHMI/Yale University School of Medicine, USA
TLR-Induced Gene Expression

Pierre Guermonprez, Kings College London, UK
Short Talk: the IFN Inducible p47 Small GTPase IGTP is Involved in Cross-presentation by MHC Class I in Dendritic Cells

Gwendalyn J. Randolph, Washington University, USA
Origins and Migratory Fate of Pulmonary Dendritic Cells

Social Hour with Lite Bites

Crestone/Red Cloud
Poster Session 2

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Crestone/Red Cloud
Longitudinal Imaging of Immune Responses

Grays Peak 2-3
Robert M. Hoffman, AntiCancer, Inc., USA
Tri-Color Imaging of the Interaction of the Host Immune System and Tumor Cells in the Living Mouse

John C. Boothroyd, Stanford University School of Medicine, USA
Dissecting Virulence and Reactivation in Toxoplasmosis Using In Vivo Imaging

* Chien Ho, Carnegie Mellon University, USA
Recent Cellular Imaging Studies Using Nano-sized Iron Oxide Particles: A New Non-invasive Approach to Detect Cardiac Transplant Rejection by MRI

Kamal M. Khanna, University of Connecticut Health Center, USA
Short Talk: Imaging the Endogenous CD8 T Cell Response to Infection: From Naïve to Memory

Matthias G. von Herrath, Novo Nordisk and La Jolla Institute for Allergy and Immunology, USA
Short Talk: Real-Time Imaging of the Pancreas During Development of Diabetes

DC in Tolerance/Immunity

Maries F. van den Broek, University Hospital Zurich, Switzerland
In Vivo Models for Studying Peripheral Tolerance

Pamela S. Ohashi, Princess Margaret Cancer Centre, Canada
Signaling Molecules Modulating Tolerance and Autoimmunity

Tim D. Sparwasser, Institute of Infection and Immunology, Germany
Short Talk: Ovalbumin-Specific Immunity in the Absence of Dendritic Cell Activation after Depletion of Regulatory T Cells in DEREG Mice

Alexander Y. Rudensky, HHMI/Memorial Sloan Kettering Cancer Center, USA
Foxp3 as the Regulatory T Cell Lineage Specification Factor

* Ira Mellman, Genentech, Inc., USA
DC Maturation: Transformation and Function

Sebastian Amigorena, Institut Curie, France
Antigen Cross Presentation in Dendritic Cells

Coffee Break

Shavano Peak Foyer
Workshop 3: Immune Imaging in Translational and Clinical Settings

Grays Peak 2-3
* Darrell J. Irvine, Massachusetts Institute of Technology, USA
Imaging Dendritic Cell Chemotaxis: Chemokine-Releasing Microspheres as Vaccine Adjuvants and Tools to Analyze Directed DC Migration

Katalin Mikecz, Rush University Medical Center, USA
Anti-CD44 Antibody Treatment, but not CD44 Deficiency, Dramatically Alters Leukocyte Recruitment in Inflamed Mouse Joints

Melanie P. Matheu, University of California, Irvine, USA
Imaging Effector Memory T Cell Response and Suppression by Potassium Channel Blockade

Tatyana Chtanova, University of California, Berkeley, USA
Visualizing the Immune Response Against T. Gondii

Heather D. Hickman, NIAID, National Institutes of Health, USA
Visualization of Early Immune Interactions After Vaccinia Virus Infection In Vivo

Tomasz Zal, University of Texas MD Anderson Cancer Center, USA
Dynamics of Endogenous Foxp3+ Regulatory T Cells in Subcutaneous and Metastatic Tumors

David A. Schaer, Memorial Sloan-Kettering Cancer Center, USA
Imaging T Cell Responses Against Melanoma

Dorian B. McGavern, NINDS, National Institutes of Health, USA
Visualizing the Immunotherapeutic Clearance of a Persistent Viral Infection

Workshop 3: Interplay between DC Subsets in Induction of Immunity and Tolerance

* William R. Heath, University of Melbourne, Australia
The Role of Dendritic Cell Subsets in Naive and Memory Responses to Infection

Alice O. Kamphorst, Emory University, USA
Antigen Targeting to CD8- DCs In Vivo

Burkhard Ludewig, Kantonsspital St. Gallen, Switzerland
Activation of Macrophages through Plasmacytoid Dendritic Cell-Derived Type I Interferon Secures Control of Murine Coronavirus Infection

Peter Siegfried Jähn, Milteniy Biotec GmbH, Germany
Functional Dichotomy of Plasmacytoid Dendritic Cells: Specific Activation of T-Cells Versus Production of Type I Interferon

Juergen M. Ruland, Technical University of Munich, Germany
CARD9 Couples Dectin-1/Syk Signaling to NF-kappaB Activation for Antifungal Immunity

Heli Uronen-Hansson, BioInvent, Sweden
Role of CD103+ DCs in the Generation of Intestinal Immune Responses

Florent Ginhoux, Singapore Immunology Network, Singapore
Developmental Regulation of Chemokines Expression Controls the Recruitment of Langerhans Cells Precursors to the Skin

Coffee Available

Shavano Peak Foyer
Application of Clinical Imaging Methods to Animal Models

Grays Peak 2-3
Anna Moore, Michigan State University, USA
In Vivo Imaging of Inflammation in Type 1 Diabetes

* Diane Mathis, Harvard Medical School, USA
Imaging beta-Cell Destruction in situ

David Piwnica-Worms, Washington University at St. Louis, USA
Molecular Imaging: Strategies to Probe Signal Transduction in Immune Responses In Vivo

DC Instruction of Adaptive Immunity

Muriel Moser, Université Libre de Bruxelles, Belgium
Injection of anti-CTLA4 mAb in primed mice induces adaptive regulatory T cells with anti-inflammatory properties

* Yong-Jun Liu, Sanofi, USA
Th Instruction by DCs

Philipp Alberts, Northern Biologics, Canada
Short Talk: Dendritic Cells Control the Expression of a VAMP8 Positive Membrane Compartment to Allow for Regulated IL12 Release

Edith Margarethe Janssen, La Jolla Institute for Allergy and Immunology, USA
Viral Modification of DC Function

Social Hour with Lite Bites

Crestone/Red Cloud



DC in Autoimmunity and Allergy

Diane Mathis, Harvard Medical School, USA
DC in Diabetes

Irah King, McGill University, Canada
Short Talk: GM-CSF Triggers Accelerated Myelopoiesis During EAE

* Fiona M. Powrie, University of Oxford, UK
Immune Regulation in the Intestine: A Balancing Act between Effector and Regulatory T Cells

Dale T. Umetsu, Genentech, Inc., USA
TIM-1 and the Development of Atopy

Bart N. Lambrecht, VIB, Ghent University, Belgium
DC in Asthma

Kristin V. Tarbell, Amgen, USA
Short Talk: DC-Expanded, Islet-Specific, Regulatory T Cells Increase Endogenous Foxp3+ Cells and Restore Normoglycemia in Diabetic NOD Mice

Workshop 4: DC in Tolerance Induction

* Matthew L. Albert, Genentech, Inc., USA
Antigen Persistence is Required for Efficient Cross-Priming of Minor Histocompatibility Antigen Specific CD8+ T Cells

Michael Y. Gerner, NIAID, National Institutes of Health, USA
Defective Direct Presentation by DC Limits CD4 T Cell Help and Causes Tolerogenic Anti-Tumor CD8 T Cell Responses

Anne Hosmalin, INSERM U1016 Institut Cochin, Université Paris Descartes, France
Human Plasmacytoid Dendritic Cells Perform Cross-Presentation

Cornelius Maihoefer, Ludwig Maximilians University Munich, Germany
RIG-I-Ligands in Tumor Therapy

Phillip J. Sanchez, National Jewish Medical and Research Center, USA
The Expression of CD70 on Dendritic Cells in vivo and its Role in Primary and Memory CD8+ T Cell Responses

Connie M. Krawczyk, Van Andel Research Institute, USA
The Effect of Jagged Expression on Dendritic Cell-Mediated Th Differentiation

Jan C. Dudda, Twincore, Germany
The Way of Regulatory T Cells from Thymus to Periphery is Imprinted by Tissue-Specific Dendritic Cells and Crucial for the Suppression of Autoimmunity

DC in Cancer

A. Karolina Palucka, The Jackson Laboratory for Genomic Medicine, USA
DCs as Vectors and Targets in Cancer Immunotherapy

Pierre G. Coulie, University of Louvain, Brussels, Belgium
Possible Mechanism of Tumor Regression Following MAGE Vaccination of Melanoma Patients.

* Carl G. Figdor, Radboud University Medical Center, Netherlands
DC Cell Immunotherapy: Monitoring, Migration and Targeting

Lieping Chen, Yale University, USA
B7-H1/PD-1 Pathway in Immune Tolerance

Concluding Keynote Address

Ralph M. Steinman, Rockefeller University, USA
Dendritic Cells and Vacine Biology

*Session Chair †Invited, not yet responded.

We gratefully acknowledge support for this conference from:

Directors' Fund

These generous unrestricted gifts allow our Directors to schedule meetings in a wide variety of important areas, many of which are in the early stages of research.

Click here to view all of the donors who support the Directors' Fund.

We gratefully acknowledge the generous grant for this conference provided by:

National Institutes of Health

Grant No. 1R13AI074292-01

We gratefully acknowledge additional support for this conference from:

JDRF UCB Celltech

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